Protein Info for GFF4533 in Sphingobium sp. HT1-2

Annotation: Na+-driven multidrug efflux pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 transmembrane" amino acids 62 to 85 (24 residues), see Phobius details amino acids 98 to 119 (22 residues), see Phobius details amino acids 139 to 159 (21 residues), see Phobius details amino acids 171 to 192 (22 residues), see Phobius details amino acids 200 to 223 (24 residues), see Phobius details amino acids 254 to 274 (21 residues), see Phobius details amino acids 293 to 311 (19 residues), see Phobius details amino acids 331 to 355 (25 residues), see Phobius details amino acids 368 to 389 (22 residues), see Phobius details amino acids 396 to 417 (22 residues), see Phobius details amino acids 427 to 447 (21 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 26 to 428 (403 residues), 207.7 bits, see alignment E=1.3e-65 PF01554: MatE" amino acids 26 to 187 (162 residues), 98 bits, see alignment E=2.5e-32 amino acids 255 to 416 (162 residues), 69.7 bits, see alignment E=1.2e-23

Best Hits

Swiss-Prot: 36% identical to YOEA_BACSU: Probable multidrug resistance protein YoeA (yoeA) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 84% identity to sjp:SJA_C1-25050)

Predicted SEED Role

"Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (483 amino acids)

>GFF4533 Na+-driven multidrug efflux pump (Sphingobium sp. HT1-2)
MAAGVQGARDLTQGPIASTLLTFAIPTLASNILQSLNGSINAIWVGRFLGPQALAATANA
NIIMFLMFSVVFGFGMASTVMIAQAVGARDMDAARRAFGSAVGFCFLLAMGVAVAGWFGG
PALLHLLATPPEAFDMALTYLRVIFVAMPASLLSVMMMMGLRGTGDARTPLIFMILSVAI
DLVLNPVLILGLGPIPAMGIGGSAAATAAAGFVSLIALVIYTYAKDLPLRLRGAELGYLR
PAMDEMRVLSTKGLPMGLQMIVMSTSGLVMIGLVNREGFLVTAAYGAAQQIWTYLQMPAM
AMGAAVSAMAAQNIGAGRWDRISRITGYGISYLLAITGAMVVVILIFHEALLALFLSHNQ
AVIDAAWNMQLLASWSFMLFGCTMILFGVMRANGVVVAPLLILVFTLFAVRLGFYYLAYP
RIGADALWLSFPVGSAVSLTLAALVYWQGGWRKAKLMVPMHEEECREAVHSETEPAGRIA
PTG