Protein Info for GFF4524 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Phosphoribosylamine--glycine ligase (EC 6.3.4.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02844: GARS_N
  • amino acids 1 to 96 (96 residues), 118.7 bits, E=2.7e-38
• TIGR00877: phosphoribosylamine--glycine ligase
  • amino acids 1 to 421 (421 residues), 527.5 bits, E=1.2e-162
• PF01071: GARS_A
  • amino acids 97 to 294 (198 residues), 252.5 bits, E=4.3e-79
• PF02843: GARS_C
  • amino acids 329 to 419 (91 residues), 103.2 bits, E=1.1e-33

Best Hits

Swiss-Prot: 69% identical to PUR2_RALSO: Phosphoribosylamine--glycine ligase (purD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 76% identity to adn:Alide_2150)

MetaCyc: 62% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Protein Sequence (421 amino acids)

>GFF4524 Phosphoribosylamine--glycine ligase (EC 6.3.4.13) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VIGGGGREHALAWKLAQADGVQQVFVAPGNAGTARDPVLTNLDITDKAALRAWAQGQGIA
LTVVGPEAPLAAGVVDEFRAHGLPIFGPTKAAAQLESSKAFSKAFMQRHGIPTAKYQTFT
DPAAAHAYVDAEGAPIVIKADGLAAGKGVVVAMTVAEAHEAIDFMLVDNTLGVAHNEGGA
RVVIEEFLVGEEASFIVLCDGEHALALATSQDHKRLMDADQGPNTGGMGAYSPAPVVTPA
VHERAMNEVILPTLRGMKADGIPYTGFLYAGLMITPDGQVKTLEFNCRMGDPETQPIMMR
LRSNLAQVLLSATRGELDRVALDWDPRVALGVVLAAAGYPLSPRKGDAISGLPADEADRM
VFHAGTSGAAQGAVQVSGGRVLCVTALADTVAGARQRAYEVVDAIHFDGMQCRRDIGYRA
L