Protein Info for PS417_23145 in Pseudomonas simiae WCS417

Annotation: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 99% identical to ISPG_PSEFS: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 99% identity to pfs:PFLU5057)

MetaCyc: 72% identical to (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (Escherichia coli K-12 substr. MG1655)
RXN-15878 [EC: 1.17.7.3]

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
taxadiene biosynthesis (engineered) (11/13 steps found)
methylerythritol phosphate pathway I (8/9 steps found)
methylerythritol phosphate pathway II (8/9 steps found)
isoprene biosynthesis I (8/10 steps found)
superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1 or 1.17.7.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UKK4 at UniProt or InterPro

Protein Sequence (369 amino acids)

>PS417_23145 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (Pseudomonas simiae WCS417)
MHGESPIKRRVSRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAATVAQINRLEAAGVDIVR
VSVPDMDAAEAFGRIKQLVKVPLVADIHFDYKIALRVAELGVDCLRINPGNIGREDRVRA
VVDAARDRGIPIRIGVNAGSLEKDLQKKYGEPTPAALVESALRHVEHLERLNFQDFKVSV
KASDVFMAVEAYRLLAKEIVQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISL
AADPVEEVKVGYDILKSLRLRSRGINFIACPSCSRQNFDVVKTMNDLELRLEDLLVPLDV
AVIGCVVNGPGEAKEAHVGLTGGTPNLIYIDGKPSQKLTNDNLVDELEKLIREKAAEKVA
ADAALIARG