Protein Info for GFF4503 in Xanthobacter sp. DMC5

Annotation: putative secretion system apparatus ATP synthase SsaN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 TIGR01026: ATPase, FliI/YscN family" amino acids 19 to 434 (416 residues), 411.1 bits, see alignment E=5.4e-127 TIGR03498: flagellar protein export ATPase FliI" amino acids 35 to 447 (413 residues), 611.7 bits, see alignment E=5.6e-188 PF00006: ATP-synt_ab" amino acids 159 to 369 (211 residues), 257.1 bits, see alignment E=1.3e-80 PF18269: T3SS_ATPase_C" amino acids 377 to 439 (63 residues), 51.2 bits, see alignment E=9.2e-18

Best Hits

Swiss-Prot: 57% identical to FLII_AGRFC: Flagellum-specific ATP synthase (fliI) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02412, flagellum-specific ATP synthase [EC: 3.6.3.14] (inferred from 76% identity to azc:AZC_0646)

Predicted SEED Role

"Flagellum-specific ATP synthase FliI" in subsystem Flagellar motility or Flagellum

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (450 amino acids)

>GFF4503 putative secretion system apparatus ATP synthase SsaN (Xanthobacter sp. DMC5)
MSAAPVNKSPMNALQRLRSAVESGMEEHPLVRVGGIVREVAPTHVRVSGLSSFVTLGERV
GFEQASGTSIGEVVRIDAAGAVVKPFDERVSVGIGTPAFRIGPLALSPDRSWKGRVIDAL
GRPLDKPEPLAHGPRRMPLDSEPPPAMARGRVHRPLVTGVRAVDIFTPICEGQRIGIFAG
SGVGKSTLLSMFARSAGFDTVVVALVGERGREVREFLEGPLAASRDRAVVIVSTSDQSPM
MRRLAPQAALAVAEYFRNAGESVLLIVDSVTRYAHAARDVALAAGEPAVARGYAPSVFST
LPKLLERAGPGMEGEGAITGIFSVLVDGDDHNDPVADAIRGTLDGHIVLDRAIADQGRFP
AINLLGSISRLAGEVWSEPERDLVRRLKAMIARFEDTSDLRLMGGYQPGADGELDQAVAI
VPRIYDALRQDPGDPPSLAAFSDLAAALKA