Protein Info for GFF4503 in Sphingobium sp. HT1-2

Annotation: Chaperone protein ClpB (ATP-dependent unfoldase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 679 PF00004: AAA" amino acids 14 to 144 (131 residues), 46.1 bits, see alignment E=2.5e-15 amino acids 410 to 528 (119 residues), 36.5 bits, see alignment E=2.2e-12 PF17871: AAA_lid_9" amino acids 152 to 254 (103 residues), 122.5 bits, see alignment E=2.4e-39 PF07724: AAA_2" amino acids 404 to 568 (165 residues), 226.9 bits, see alignment E=5.2e-71 PF07728: AAA_5" amino acids 409 to 529 (121 residues), 45.4 bits, see alignment E=3e-15 PF10431: ClpB_D2-small" amino acids 574 to 654 (81 residues), 89.9 bits, see alignment E=3.2e-29

Best Hits

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (679 amino acids)

>GFF4503 Chaperone protein ClpB (ATP-dependent unfoldase) (Sphingobium sp. HT1-2)
VQILARRTKNNPALMGEPGVGKTAIAEGLALRIANGDVPESLRDRRLMALDMGSLIAGAK
YRGEFEERLKSVLDEVKGAEGDIILFIDEMHTLIGAGKGEGAMDASNLLKPALARGELHC
IGATTLDEYQKYVEKDPALQRRFQPVFVGEPTVEDTISILRGIKENYELHHGVRIADNAI
VAAATLSNRYIADRFLPDKAIDLMDEAASRIRMEVESKPEEIEGLDRRIIQLKIEEQALA
KETDQASRDRLEALREDLANLEQQSAELTTRWQNERDKIASEGKIKEQLDAARVELEQAQ
RAGDYAKAGELTYSRIPALEKSLEDAQGQSNNALLREEVTSEDIAAVVSRWTGIPVDRMM
EGERDKLLKMEEVIGKRVIGQKDAVLAVSKAVRRARAGLQDPNRPLGSFLFLGPTGVGKT
ELTKALAGFLFDDDAAMVRIDMSEFMEKHSVSRLIGAPPGYVGYDEGGVLTEAVRRRPYQ
VVLFDEVEKAHGDVFNVLLQVLDDGRLTDGQGRVVDFTNTLIILTSNLGSQFLANLGDGE
DVEKVEPQVMDVVRGHFRPEFLNRLDEIILFHRLGQEHMGPIVEIQVARVGKLLKDRKIT
LDLTEAAERWLGRIAYDPVYGARPLKRAVQRYLQDPLAEKLLAGEIPDGARVRIDEGDDG
LLFEVDAGLRADRREPQAA