Protein Info for PS417_23035 in Pseudomonas simiae WCS417

Updated annotation (from data): D-trehalose PTS system, I, HPr, and IIA components
Rationale: Specific phenotype for trehalose and no other trehalose transporter is apparent in the fitness data. The IIB and IIC components are provided by PS417_23050
Original annotation: PTS mannose transporter subunit IIC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 838 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 638 to 658 (21 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 8 to 129 (122 residues), 141.2 bits, see alignment E=3.6e-45 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 9 to 127 (119 residues), 128 bits, see alignment E=2.9e-41 TIGR01003: phosphocarrier, HPr family" amino acids 169 to 247 (79 residues), 72.8 bits, see alignment E=2.7e-24 PF00381: PTS-HPr" amino acids 169 to 248 (80 residues), 90.6 bits, see alignment E=1.4e-29 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 272 to 820 (549 residues), 506.7 bits, see alignment E=1.1e-155 PF05524: PEP-utilisers_N" amino acids 272 to 388 (117 residues), 67.7 bits, see alignment E=2.6e-22 PF00391: PEP-utilizers" amino acids 414 to 486 (73 residues), 75.9 bits, see alignment E=3.9e-25 PF02896: PEP-utilizers_C" amino acids 517 to 798 (282 residues), 338.7 bits, see alignment E=7e-105

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 92% identity to pfs:PFLU5037)

Predicted SEED Role

"PTS system, glucose-specific IIA component (EC 2.7.1.69) / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Trehalose Uptake and Utilization or Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.1.69, EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UBM9 at UniProt or InterPro

Protein Sequence (838 amino acids)

>PS417_23035 D-trehalose PTS system, I, HPr, and IIA components (Pseudomonas simiae WCS417)
MTTTQPLELLAPLSGVLLALDKVPDPVFSSRLIGDGLCIDPTSQTLCAPLAGVISNIQDS
GHAVSITDDNGVQVLMHIGLDTVNLAGQGFTRLVQEGQRVEAGQPLIEFDADYVALNARS
LLTLMLVVSGEPFSLLADGLVETGQPLLQLSPSGAVEAVDEEEGDALFSKPLTLPNANGL
HARPAAVFAQAAKGFNASIYLHKQTQSANAKSLVAIMALQTVQGDTLQVSAAGEDAEAAI
KALVALLAEGCGEAVVNVAEPVATQSSATLLRGVCASPGSAFGQVVQVTDPELVITEQGT
GGATERAALTRGLLAANEALQVLQDKAAGSAQAEIFRAHQELLEDPTLLEHAHRLLGEGK
SAAFAWNSATLATVTLFQGLGNALIAERAADLADVGQRVLKLILGIQDSAWDLPERAILI
AEQLTPSQTASLDTRKVLGFVTVAGGATSHVAILARALGLPAICGVPAQVLALANGKQVL
LDADKGELHLEPNLAEIEQLEAARKHQVLRHQRDVAQASLPATTRDGHHVEVTANVASLQ
EVEHALTLGGEGVGLLRSEFLYLDRNRAPSPEEQAGTYTAIARALGTERNLVVRTLDVGG
DKPLAYVPMDAETNPFLGLRGIRLCLERPQLLREQFRAILASAGFARLHIMLPMVSLLSE
LHLARKILEEEALALGLTELPKLGIMIEVPSAALMADVFAPHVDFFSIGTNDLTQYTLAM
DRDHPRLANQADSFHPAVLRLIATTVKAAHAHGKWVGVCGALASEALAVPVLIGLGVDEL
SVSVPLIPTIKATVRELDLADCQIIARQVLGLEEAAEVREALRQYHAATVESSPVVEH