Protein Info for GFF45 in Variovorax sp. SCN45

Annotation: Phospholipase C (EC 3.1.4.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details TIGR03396: phospholipase C, phosphocholine-specific" amino acids 4 to 754 (751 residues), 951.5 bits, see alignment E=2.9e-290 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 4 to 26 (23 residues), 18.4 bits, see alignment (E = 2.1e-07) PF04185: Phosphoesterase" amino acids 47 to 485 (439 residues), 354.8 bits, see alignment E=5.8e-110 PF05506: PLipase_C_C" amino acids 557 to 646 (90 residues), 97.4 bits, see alignment E=7.7e-32 amino acids 658 to 740 (83 residues), 85 bits, see alignment E=5.6e-28

Best Hits

Predicted SEED Role

"Phospholipase C 4 precursor (EC 3.1.4.3)" (EC 3.1.4.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.3

Use Curated BLAST to search for 3.1.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (758 amino acids)

>GFF45 Phospholipase C (EC 3.1.4.3) (Variovorax sp. SCN45)
MKNSRRNFLKGTASSGLAAVALTAFPPSIRRALAIPANNKTGTIMDVEHVVILMQENRSF
DHYFGTLMGVRGFGDRFTIPLPKGMNVWQQSDSAGKPVLPYHLDQSKGNAQRVSGTPHSW
GDAQNAWDGGRMYQWPRYKNTASMGYFMQAELPFQYALANAFTLCDAYHCGMHTGTNSNR
LFHWSGTNGPSGQVGTGLAGTASIDNTWENLRYPWNPDTDPNGDSTGYGCDWKTYPERLE
EAKVSWIVYQNLPDNYTDNPLHGFKQYRMANKVSGKNKMCYDGDASPPAGQTVTAYDPAS
DDAGNPLYKGVANTMPDGGFLGTFKQDVANGKLRQVSWIVAPATYSEHPGPSSPVQGAWY
IQEVLDALTAYPDVWSKTVFIVNFDENDGYFDHMPSPSAPSLIDPTNPTGAVAGKTTLSD
ADVAFERHTYPLVGSTTVPSDGRVYGPGPRVPMYVISPWSRGGWVNSQVFDHTSVLRFLE
ARFGVREMNISPYRRAIAGDLTSAFNFVSPNNDALPALAGRMTLAQANQLRADQQALPQI
PVPLDPQLPRQATGTRPSRALPYELHTSARSDVVNGKLQLLFANTGKAAAVFHVYDKLNL
GLLPRRYMVEAGKTLDDTWSPMADNSGFYDLWVLGPNGYHRHFKGNLNTLRAGDVAVPEV
RVCYDIANGNVYLKMLNGGKNACKFTIRAKAYRDDGPWTATVSGGTVAEQHWDLSASGCW
YDFAVTCDSDPSYYRRFAGRVETGRHSVSDPAMGMADL