Protein Info for PS417_23020 in Pseudomonas simiae WCS417

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1298 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1297 (1295 residues), 2131.1 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 148 (114 residues), 133.7 bits, see alignment E=7.5e-43 PF18072: FGAR-AT_linker" amino acids 169 to 218 (50 residues), 83.7 bits, see alignment (E = 2.6e-27) PF02769: AIRS_C" amino acids 430 to 587 (158 residues), 115.7 bits, see alignment E=5.9e-37 amino acids 839 to 967 (129 residues), 56 bits, see alignment E=1.3e-18 PF22689: FGAR-AT_PurM_N-like" amino acids 646 to 805 (160 residues), 282.4 bits, see alignment E=2.3e-88 PF13507: GATase_5" amino acids 1042 to 1297 (256 residues), 367.7 bits, see alignment E=6.6e-114

Best Hits

Swiss-Prot: 66% identical to PUR4_HAHCH: Phosphoribosylformylglycinamidine synthase (purL) from Hahella chejuensis (strain KCTC 2396)

MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UMY5 at UniProt or InterPro

Protein Sequence (1298 amino acids)

>PS417_23020 phosphoribosylformylglycinamidine synthase (Pseudomonas simiae WCS417)
MLILRGAPALSAFRHSKLLEQLSQKVPAVTGLYAEFAHFADVTGVLTADEQQVLARLLKY
GPSVPVQEPNGRLFLVLPRFGTISPWSSKASDIARNCGLEKIQRLERGIAFYVAGQFSDA
EAELIASSLHDRMTQIIVSQLEQAGGLFSHAEPKPLTAIDVLGGGRAALEKANTELGLAL
AEDEIDYLVNAFNGLKRNPHDIELMMFAQANSEHCRHKIFNASWDIDGESQEKSLFGMIK
NTYVMHSEGVLSAYKDNASVIVGSVAGRFFPDPETRQYGAVQEPVHILMKVETHNHPTAI
APFPGAATGSGGEIRDEGATGRGAKPKAGLTGFTVSNLQIPGFEQPWEVPYGKPDRIVTA
LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQSITTPRGDEVRGYHKPIMLAGGMGNIRE
EHVKKGEILVGSKLIVLGGPAMLIGLGGGAASSMATGTSSADLDFASVQRENPEMERRCQ
EVIDRCWQLGDKNPISFIHDVGAGGLSNAFPELVNDGDRGGRFELRNIPNDEPGMAPHEI
WSNESQERYVLAVGAEDFARFQAICERERCPFAVVGEATAEPQLTVTDSHFGNSPVDMPL
EVLLGKAPRMHRSAVREAELGDDFDPSQLELADSIERVLHHPAVASKSFLITIGDRTITG
LVARDQMVGPWQVPVADVAVTATSFDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTN
IAASRIGKLSDIKLSANWMSAAGHPGEDARLYDTVKAVGMELCPELGITIPVGKDSMSMA
TRWNEDGVDKTVTSPLSLIVTGFAPVTDIRQTLTPQLRMDKGTTDLILIDLGRGQNRMGA
SILAQTHGKLGKQAPDVDDAEDLKAFFAVIQGLNADGHLLAYHDRSDGGLLTSVVEMAFA
GHCGLNIVLDSVAEDASEINGILFNEELGAVIQVRQDATPDVLAQFSAAGLDDCVAVIGQ
PINNGEINISFNGDTVFAGQRRLLQRQWAETSYQIQRLRDNADCAEQEFDVILEEDNPGL
STKLSFDVNQDIAAPYIKKGIRPQVAVLREQGVNGQVEMAAAFDRAGFNAIDVHMSDILA
GRVDLNAFKGLVACGGFSYGDVLGAGEGWAKSALFNSRARDAFQGFFERNDSFTLGVCNG
CQMMSNLSELIPGSEFWPHFVRNRSEQFEARVAMVQVQESNSIFLQGMAGSRMPIAIAHG
EGHAEFASEEALLEADLSGTVALRFVDNHGKVTETYPANPNGSPRGITGLTSRDGRVTIM
MPHPERVFRAVQNSWRPEEWNEDGAWMRMFRNARVWVN