Protein Info for GFF4494 in Sphingobium sp. HT1-2

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 789 transmembrane" amino acids 134 to 155 (22 residues), see Phobius details amino acids 167 to 186 (20 residues), see Phobius details amino acids 198 to 221 (24 residues), see Phobius details amino acids 236 to 256 (21 residues), see Phobius details amino acids 391 to 413 (23 residues), see Phobius details amino acids 419 to 442 (24 residues), see Phobius details amino acids 735 to 756 (22 residues), see Phobius details amino acids 761 to 782 (22 residues), see Phobius details PF04945: YHS" amino acids 30 to 75 (46 residues), 46.2 bits, see alignment 9.3e-16 PF19335: HMBD" amino acids 89 to 115 (27 residues), 53 bits, see alignment (E = 6.5e-18) TIGR01511: copper-translocating P-type ATPase" amino acids 181 to 784 (604 residues), 595.6 bits, see alignment E=2.2e-182 TIGR01525: heavy metal translocating P-type ATPase" amino acids 200 to 784 (585 residues), 634.1 bits, see alignment E=5e-194 TIGR01494: HAD ATPase, P-type, family IC" amino acids 245 to 754 (510 residues), 300 bits, see alignment E=4.8e-93 PF00122: E1-E2_ATPase" amino acids 274 to 454 (181 residues), 197.5 bits, see alignment E=3.5e-62 PF00702: Hydrolase" amino acids 472 to 689 (218 residues), 129.4 bits, see alignment E=5.6e-41

Best Hits

Swiss-Prot: 69% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 79% identity to sjp:SJA_C1-04830)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (789 amino acids)

>GFF4494 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Sphingobium sp. HT1-2)
VTDPVPASNSGAHSCCAAKHGGGTDAKTAKDPVCGMMVDPAATPHHASHHGEQYHFCSAG
CRTKFVAEPVRYLSAGDKEAMVAQPGAIWTCPMHPEVRQDHPGPCPICGMALEPELVTAD
TGPSGELLDMSRRFWIAVLLTIPVFVLEMGSHLFPALHNLVPARMSGWLQLALATPVVLW
AGWPFFERGWVSIRTRNLNMFTLIAMGTGVAWAYSVVATIAPRVFPAAFRMMDGTVAIYF
EAAAVITVLVLLGQVLELRARERTSGAIKALLNLAPKTARRIGADGNDEEVSLDQVAVGD
LLRVRPGEKVPVDGKVEDGRSSLDESMVTGESMPVTKALGDTVIGGTINQSGALVMRAEK
VGRDTMLARIVQMVADAQRSRAPIQRLADQVAGWFVPAVLLIALIAFGVWGIWGPEPRLA
HGLIAAVAVLIIACPCALGLATPMSIMVGIGRGAGLGVLIKNAEALERMEKIDTLVVDKT
GTLTEGRPSVTRIIPATGYEETELLRLAAGVERASEHPLALAVIAAAKDRGIVLPEVADF
DSPTGKGAIGTVDGKRITLGNARFLSENGIDVSALEPQADELRQDGATAIFIGIDKQAAG
ILAIADAIKATTAEAIAALRKEGIRVVMLTGDNRTTAEAVGRKLGIDDVEAEVLPDRKSA
VVARLKSEGRIVAMAGDGVNDAPALAAADVGIAMGSGTDVAIESAGVTLLKGDLNGIVRA
RRLSEATMSNIRQNLFFAFIYNAAGIPIAAGLLYPIFGILLSPIIAAAAMALSSVSVVTN
ALRLNRAAL