Protein Info for PS417_22995 in Pseudomonas simiae WCS417

Annotation: PTS N-acetyl-D-glucosamine transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 836 PF00358: PTS_EIIA_1" amino acids 9 to 132 (124 residues), 148.3 bits, see alignment E=2.4e-47 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 11 to 130 (120 residues), 131.4 bits, see alignment E=2.5e-42 PF00381: PTS-HPr" amino acids 173 to 252 (80 residues), 82.9 bits, see alignment E=3.4e-27 TIGR01003: phosphocarrier, HPr family" amino acids 173 to 251 (79 residues), 72.5 bits, see alignment E=3.2e-24 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 278 to 827 (550 residues), 468.1 bits, see alignment E=5.5e-144 PF05524: PEP-utilisers_N" amino acids 278 to 397 (120 residues), 72.3 bits, see alignment E=9.9e-24 PF00391: PEP-utilizers" amino acids 422 to 494 (73 residues), 65.4 bits, see alignment E=7.2e-22 PF02896: PEP-utilizers_C" amino acids 520 to 805 (286 residues), 343.1 bits, see alignment E=3.1e-106

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 93% identity to pfs:PFLU5027)

Predicted SEED Role

"PTS system, glucose-specific IIA component (EC 2.7.1.69) / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Trehalose Uptake and Utilization or Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.1.69, EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UR78 at UniProt or InterPro

Protein Sequence (836 amino acids)

>PS417_22995 PTS N-acetyl-D-glucosamine transporter (Pseudomonas simiae WCS417)
MSYNNNELTLSAPLSGPVLTLGNVPDEVFASGAMGDGIAIDPLNDCLYAPCDGVIIHVAR
TGHALTIRAENGAEVLMHVGIDTVELNGEGFALLVKDGARVSKGQALVQFDLDRIARQCK
SLVSLIILTNGERFELRAVAGQKVKVGEPLLQIVARSAAAVQTAVDNSEADVSASVRITH
RGGLHARPAALIRKTAQDFSSQAQLHFGDKSASCDSLIGLMGLGIGEGDEVRVTCRGKDA
EAALQALVAALSAVIKEEHHVPVVAPPRRAHTEAGVLQGVCAAPGLVCGPLFRLTGIELP
ADVGNHSADEQLQHLDTALEQVRSEIRSTLAHARQRKNVEEEDIFAAHLALLEDPNLLEA
ATRSIEQGSAATHAWRDAIQAQCAVLLALGKPLFAERANDLRDLQQRVLRALLGEAWHFE
LPAGSIVSAHELTPSDLLQLSAQHAVGICMAEGGATSHVAILARGKGLPCVVALGAEVLD
VPQGQRVVLDAVNGRLELEPTEARHAEVHQIRDAQKLRRQQQQAQAQEPARTTDGVSIEV
AANVASSAEAQVAFENGADGVGLLRTEFLFVDRRTAPDEQEQRHAYQAVLDAMGDKSVII
RTIDVGGDKQLDYLPLPVEANPVLGLRGIRMAQVRPELLDQQLRALLQVSPLERCRILLP
MVSEVDELLQIRQRLDELCVELELTQRPELGVMIEVPAAALMAEHLAKHADFLSIGTNDL
SQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGATKHGRWVGVCGALASDPLATPVLV
GLGVSELSVSPPQIAEIKDRVRHLDAAQCRQLSQGLLDLSSAKAVRQACQHHWPLS