Protein Info for GFF449 in Sphingobium sp. HT1-2

Annotation: FIG00824362: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K07749, formyl-CoA transferase [EC: 2.8.3.16] (inferred from 32% identity to cak:Caul_0077)

Predicted SEED Role

"FIG00824362: hypothetical protein"

MetaCyc Pathways

oxalate degradation II (1/3 steps found)

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.16

Use Curated BLAST to search for 2.8.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (399 amino acids)

>GFF449 FIG00824362: hypothetical protein (Sphingobium sp. HT1-2)
MNRGGPLAGVRVLEIAKVWAGPYAGKLLAFLGAEVIKVESRANLDEMRAYGGVDIDSAPY
FRSINQEVLSVQLDMKSDEGMTHLRAMIAASDVLIDNLRPGAMERSGLGYEDVRAIRPDI
IQLSIKMYGSDGPLGYQTGYAPCFAALGGLNALVGHVGETPAGMNIRYGDSTVGAAAALA
IVAALHHRETTGEGQFIDLSAVETLSMMVGDSLFAYGLAGELPQATGNAHADMAPHGCYP
CADGQWISLAIADDREWTAFCATVDAQALAVDPLFVHASDRLANRDALDAALTQRSPDWD
AEAMAQVLLLAGVPAFRSQSSLDLIGSEALWALGAYRMVSDAKGEPRPIIGPGWRMTPDE
ASITRGAPLLGEHNHLVYGEILGLSDSEIDDLIARRVIW