Protein Info for GFF4482 in Xanthobacter sp. DMC5

Annotation: Flagellar M-ring protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 437 to 456 (20 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 19 to 553 (535 residues), 220.1 bits, see alignment E=3.6e-69 PF01514: YscJ_FliF" amino acids 44 to 217 (174 residues), 189.2 bits, see alignment E=6.4e-60 PF08345: YscJ_FliF_C" amino acids 249 to 412 (164 residues), 117.9 bits, see alignment E=5.4e-38

Best Hits

Swiss-Prot: 50% identical to FLIF_BRUSU: Flagellar M-ring protein FliF (fliF) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 73% identity to azc:AZC_0627)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>GFF4482 Flagellar M-ring protein (Xanthobacter sp. DMC5)
MIGKEQLEQLWRNLRDLGARRLIALALIGLTAVAAVALGAWYLGQPEREPLYANLTREDV
NRIGGALKDAGIPFDVNADGTTVLVSHSDTARARMLLAVKGLPQSGNTGYELFNDVRAFG
LTSFMQEVTRVRALEGELARTIQTMKGIKAARVHIVLPERGSFRRDQQSASASVVIRTET
AEDASTAQAIRHLVAAAIPGLKMDQVTVLSTEGAVLSSSEDGASAAAGKKAMLQQQVSRE
TEEKVRRTLAPYIGLGNFEVQVSARLDTDRVTTSETTFDPASKVERSVRVVKELGQSQNK
TKQPNTSVQQNIPGQTQQGDGGTDSNENSSRREELTNFEVSTRTVQTVREAFVVRGLSIA
VLVNKERLMGAAGGADAIPVEQQLYEVEQLVASAAGFDKERGDKLKVAAVSFANGGAALE
PVPPLPWSELLLRQAGTMINAATILIVAGLVIWFGLKPAVRAILARPERDEVEDAEAIEA
AMLAGGAPLAAVAGPSMVPGEGAAGEEAPGALSLIEDLTSRLNRSPQKRLEQIVEFDEEQ
VAAILRQWLHQDARA