Protein Info for GFF4477 in Variovorax sp. SCN45

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alani ne ligase (EC 6.3.2.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 31 to 99 (69 residues), 22.3 bits, E=2.1e-08
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 36 to 465 (430 residues), 387.8 bits, E=3.3e-120
• PF08245: Mur_ligase_M
  • amino acids 113 to 308 (196 residues), 136.3 bits, E=2.1e-43
• PF02875: Mur_ligase_C
  • amino acids 343 to 455 (113 residues), 57.2 bits, E=4.9e-19

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 82% identity to vpe:Varpa_0975)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.10

Use Curated BLAST to search for 6.3.2.10

Protein Sequence (482 amino acids)

>GFF4477 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alani ne ligase (EC 6.3.2.10) (Variovorax sp. SCN45)
MTDSTPLGITLAQAQQWIPGSRLVGDASTVIARVHTDTRTLAAGDLFVALKGERFDANEF
LAEAKKQGAVAAIAHHGLEAAGLAGLEVPDSLLALGALGAGWRAQFTLPLIAVTGSNGKT
TVTQMIAAVLFAARADRGESALATAGNFNNEIGVPLTLLRLRAQHQRAVLELGMNHPGEI
ARLAAMSRPTIALVNNAQREHQEFMATVEAVAVENAAVFAVLPDDGTAVFPYGDAFTSLW
TDMAHAGGSRRCMTFGEHQDADISLDGAEWKQGAWAVRIRTPIGSFDARLHIAGRHNVVN
ALAATACALAAGVSTDIIAAGLSAFEPVKGRSRASEIVLADGRAVTLVDDSYNANPDSVI
AAIDVLAALPGPRLLVLGDMGEVGERSPEFHAEVGDWARQRGIEAVYTFGNATAHTVAAF
RGDADGRHFNEFDALGAAVLARLPHVGSVLVKGSRSMKMERMVQAIAAQARPQQQKEAGH
DA