Protein Info for GFF4476 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Arsenic efflux pump protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 52 to 71 (20 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 116 to 147 (32 residues), see Phobius details amino acids 149 to 165 (17 residues), see Phobius details amino acids 176 to 198 (23 residues), see Phobius details amino acids 219 to 240 (22 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 278 to 296 (19 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details amino acids 401 to 425 (25 residues), see Phobius details TIGR00935: arsenite/antimonite efflux pump membrane protein" amino acids 3 to 425 (423 residues), 712.2 bits, see alignment E=1.4e-218 PF02040: ArsB" amino acids 4 to 423 (420 residues), 517.1 bits, see alignment E=4.9e-159 PF03600: CitMHS" amino acids 13 to 368 (356 residues), 208.4 bits, see alignment E=1.6e-65

Best Hits

Swiss-Prot: 74% identical to ARSB2_ECOLX: Arsenical pump membrane protein (arsB) from Escherichia coli

KEGG orthology group: None (inferred from 83% identity to ctt:CtCNB1_3139)

MetaCyc: 76% identical to arsenical pump membrane protein (Shewanella sp. ANA-3)
RXN-22366 [EC: 7.3.2.7]

Predicted SEED Role

"Arsenic efflux pump protein" in subsystem Arsenic resistance

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>GFF4476 Arsenic efflux pump protein (Hydrogenophaga sp. GW460-11-11-14-LB1)
MIAAVLIFAFTLLLVIWQPRGLGIGWSALLGAGVALLAGVVQLSDIPVVWDIVWNATATF
VAVIVISLVLDEAGFFEWAALHFARWGGGRGPRLFAFIVLLGAVVAALFANDGAALILTP
IVMAMLVALGFSPAATLAFVMAAGFIADTASLPLVVSNLVNIVSADFFDIGFNRYAAVML
PVNLASVAASLVVLYLYFRRDLPATYDLRQLKAPSAAIRDLNTFRAGWGVLGLLLVGFFG
LEPLGVPVSAVAAAGAAILLAVAARGHVIGTRKVLKEAPWQIVVFSLGMYLVVYGLRNAG
LTGHLAALLDRFAQGGVWGAALGTGFLAAALSSVMNNMPTVLVGALSIDQSTATGVVKEA
MVYANLIGCDLGPKITPIGSLATLLWLHVLARKGTTIAWGYYFRVGVVLTLPVLAVTLAA
LALRLGAT