Protein Info for GFF4475 in Variovorax sp. SCN45

Annotation: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (EC 6.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 706 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 522 to 543 (22 residues), see Phobius details PF21799: MurD-like_N" amino acids 8 to 99 (92 residues), 44.1 bits, see alignment E=3.1e-15 TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase" amino acids 315 to 687 (373 residues), 315.5 bits, see alignment E=3.4e-98 PF08245: Mur_ligase_M" amino acids 320 to 529 (210 residues), 92 bits, see alignment E=7.5e-30 PF02875: Mur_ligase_C" amino acids 551 to 666 (116 residues), 60.7 bits, see alignment E=4.1e-20

Best Hits

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 80% identity to vap:Vapar_0917)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (706 amino acids)

>GFF4475 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (EC 6.3.2.9) (Variovorax sp. SCN45)
MRHLKDLHVLILGLGASGLAMARWCARHGAVVTVADTREAPASLATLKAEWPEVAFVGGP
FTAALVEGTPIRAVYRSPGLSPATIAPVADAARAMGLPVGGELDLFARALLDLRTVEVPV
VEAEPEPEEAIPEAPVVEAAEPEAQAELPLEAPVPAEQAEVEVVAEPVPQPEPVAETQAL
ETVVAEAAPEPTVPAQAEAMPRDPSMSVAVPQPPETDAPVEAAPEAVEPVAETPEAPEAV
EPPAATPAPAPATVSRLPATGKPYVPTAAKDAAEFVAKIAELSATNPASAAVEEESPQLE
LAPPVEPEAPKGYTPSVLAITGTNGKTTVTSLTGQLVERGGKTVAVAGNIGPTLLDTLWA
HIDADTLPDVWVLELSSFQLDGVQDFEPTAATVLNLTQDHLDWHGDMSAYAAAKARIFGA
RGLMILNRDDAGVMAMLPAPVKVKLQRPQIRTHVTFGAAMPLRPGDYGIERVNGMAWLVR
ALEADETQKRKRGAVVEEEIFLQRLMPADALRIRGRHNAMNALAALALASAAGCPLGPML
YGLREYHGEPHRVEPIAIVDEVEYFDDSKGTNVGATVAALAGLGEERRVVVILGGEGKGQ
DFEPLAAPVRQYARAVVLIGRDASIIEAALASTGVSLMHAGSMEEAVQAAAARANPGDAV
LLSPACASFDMFRDYEHRADVFREAVQSWADSPRGLGDSSTTEEQL