Protein Info for GFF4443 in Xanthobacter sp. DMC5
Annotation: Uptake hydrogenase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to MBHL_OLICO: Uptake hydrogenase large subunit (hoxL) from Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
KEGG orthology group: K06281, hydrogenase large subunit [EC: 1.12.99.6] (inferred from 97% identity to xau:Xaut_2174)MetaCyc: 72% identical to hydrogenase large subunit (Cupriavidus necator)
Hydrogenase (acceptor). [EC: 1.12.99.6]
Predicted SEED Role
"Uptake hydrogenase large subunit (EC 1.12.99.6)" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase (EC 1.12.99.6)
MetaCyc Pathways
- hydrogen oxidation I (aerobic) (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.12.99.6
Use Curated BLAST to search for 1.12.99.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (604 amino acids)
>GFF4443 Uptake hydrogenase large subunit (Xanthobacter sp. DMC5) VTIQTPNGFSLDTSGKRIVVDPVTRIEGHMRCEVNVDSNNIIRNAVSTGTMWRGLEVILK GRDPRDAWAFVERICGVCTGCHALTSVRAVEDALQIKIPNNAFLIREIMAKVLQWHDHVV HFYHLHALDWVNPVNALKADPKATSVLQQSISAHSKSSPGYFRDVQNRLKKFVESGQLGI FKNGYWDNPAYLLPPEADLLAVTHYLEALDFQREIVKVHTIFGGKNPHPNYLVGGVPCAI NMNGDMAAGAPLNMERLNFVKLKLQEAFEFSQNVYVPDVIAIASFYKGWLYGGGLSGQNV MDYGDYEAIQGQKKTDRLPGGVILNGNWNEVHPIDPRDPAQVQEFVAHSWYTYGEGNNDK GLHPWDGVTDPQYKLGPNAKGTRTNIEAVDENAKYSWIKAPRWKGNAVEVGPLARYILAY AQGVDYVKGQVDEALGTFNTLAGTNLTAKQALPTTIGRTLARALEAHYCAKMMLDDFDHL IANIKAGDTSTANMEKWDPATWPKEAKGVGTVAAPRGGLGHWIKIKDGRIENYQCVVPTT WNGGPRDPKGNIGAFEASLMNTPMQRPEEPVEILRTLHSFDPCLACSTHVMSDDGAELAR VTVR