Protein Info for GFF4442 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Conjugative transfer protein TrbG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF03524: CagX" amino acids 100 to 319 (220 residues), 226.7 bits, see alignment E=1.4e-71 TIGR02775: P-type conjugative transfer protein TrbG" amino acids 101 to 308 (208 residues), 293.6 bits, see alignment E=3.3e-92

Best Hits

KEGG orthology group: K03204, type IV secretion system protein VirB9 (inferred from 91% identity to adn:Alide_0210)

Predicted SEED Role

"Conjugative transfer protein TrbG" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (326 amino acids)

>GFF4442 Conjugative transfer protein TrbG (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNSFFRVYVSVLTLAALAGCATQGKPPPVISLDEPVQAQPLPEAPKPVEVVAVPEVLPMP
AQMKPVPEAKPAAEPVDETMRVSRANAEARIAPTREGYVNAIQVWPFTDGALYQVYAAVG
RVTVIALQAGEELVTVAAGDTVRWIVGDTSSGNGDALRVNVMVKPIRSGLKTNLVITTSR
RTYLLELTSTEKTWMASVSWEYPKDKMLALQRQAQAAQTATPVDAGLSLEKIRFRYAIGG
SNPPWKPLRAFDDGEKVYIQFPAGIAQGELPPLFVIGAQGDGQLVNYRFRSPYYIVDRLF
GTAELRLGGDKGDVVRIERTDGTRRN