Protein Info for GFF4441 in Xanthobacter sp. DMC5

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 239 TIGR00140: hydrogenase expression/formation protein" amino acids 10 to 158 (149 residues), 184 bits, see alignment E=1.7e-58 TIGR00072: hydrogenase maturation protease" amino acids 11 to 156 (146 residues), 151.5 bits, see alignment E=1.6e-48 PF01750: HycI" amino acids 27 to 155 (129 residues), 126.7 bits, see alignment E=2.6e-41

Best Hits

Swiss-Prot: 49% identical to HUPM_AZOCH: Hydrogenase expression/formation protein HupM (hupM) from Azotobacter chroococcum mcd 1

KEGG orthology group: K03605, hydrogenase 1 maturation protease [EC: 3.4.24.-] (inferred from 83% identity to xau:Xaut_2176)

Predicted SEED Role

"Hydrogenase maturation protease (EC 3.4.24.-)" in subsystem Membrane-bound Ni, Fe-hydrogenase (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (239 amino acids)

>GFF4441 hypothetical protein (Xanthobacter sp. DMC5)
MDGERICARILVLGIGNILWADEGFGVRVLETLDRAYTFPEPVTLLDGGTQGLYLLPYLE
EAEGVVIVDAVDFGSPPGTVHIRADADVPAVLAARKVSLHQTGFQEILGLLALRGHTPGR
IVLVGVQPEVLDDYGGGLTATVAAQIEPAARKVLAILKDEFGIVPEPRAGEGSLVTAAVA
RDPYETERPSAEDACRIGDARVLVRHEGDPIGSPSESNDRERVIRSDCAAIGSARALGA