Protein Info for GFF4438 in Sphingobium sp. HT1-2

Annotation: Plasmid replication protein RepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 PF10609: ParA" amino acids 112 to 288 (177 residues), 34.5 bits, see alignment E=3.9e-12 PF09140: MipZ" amino acids 115 to 163 (49 residues), 29.4 bits, see alignment 1.3e-10 PF13614: AAA_31" amino acids 115 to 275 (161 residues), 114.6 bits, see alignment E=1.3e-36 PF01656: CbiA" amino acids 116 to 358 (243 residues), 74.8 bits, see alignment E=1.6e-24

Best Hits

KEGG orthology group: None (inferred from 88% identity to npp:PP1Y_Mpl5117)

Predicted SEED Role

"Plasmid replication protein RepA" in subsystem Plasmid replication

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (401 amino acids)

>GFF4438 Plasmid replication protein RepA (Sphingobium sp. HT1-2)
MASEQTLEAPQDELLDDGLGLLHRRALTILKRIRDNAIDPETGAKKGPSFPISKAAALVS
RTASAIREAERDGRLPARGRTASGHRVQYSLEELDHMREVFGTRPWRSPEDVPAIISVSN
FKGGVGKSTIALHLAQHFAIRGYRVLFIDCDSQASSTMMFGYRPDIDLSEEDTLYGHFHN
PELLGVRKIIRKTHFFGLDLIPANLKLYNLEYEIAGYLAQNQSFDIIDMIAQAIDDVVDD
YDVVIMDPPPALGMVSMAVLQAANAMVIPVPPSVIDFASTVSFIDMARTTMRQLEKLAGR
VKPAYNFIRLVGSRVDESKSMQSEILTMMRQVFGGSMISSVLRTSAEIDNASSRMKTVFE
LDRPVTSHEVHNRCVKYLNEVCLDIEKDVLRTWQSRAGEVA