Protein Info for GFF4433 in Xanthobacter sp. DMC5

Annotation: Hydrogenase transcriptional regulatory protein hupR1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 PF00072: Response_reg" amino acids 7 to 115 (109 residues), 80.2 bits, see alignment E=3.9e-26 PF14532: Sigma54_activ_2" amino acids 170 to 335 (166 residues), 73.6 bits, see alignment E=5.9e-24 PF00158: Sigma54_activat" amino acids 171 to 330 (160 residues), 224.9 bits, see alignment E=1.6e-70 PF07728: AAA_5" amino acids 187 to 307 (121 residues), 31.4 bits, see alignment E=5.4e-11 PF02954: HTH_8" amino acids 440 to 480 (41 residues), 44.7 bits, see alignment 2.6e-15

Best Hits

Swiss-Prot: 54% identical to HOXA_BRADU: Hydrogenase transcriptional regulatory protein HoxA (hoxA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: None (inferred from 91% identity to xau:Xaut_2184)

Predicted SEED Role

"Hydrogenase transcriptional regulatory protein HoxA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (491 amino acids)

>GFF4433 Hydrogenase transcriptional regulatory protein hupR1 (Xanthobacter sp. DMC5)
MNRRPAILVVDDEPRSVEVIARVLDEEFDVFTALNAEEARRILENEWIQVIFCDQRMPQT
SGVALLTEVRERWPDILRIIVTGYTEPEDMIGAINDAGIYQFIPKPWHPDQLLLAARNAA
HLFQLQRDHERLNLEMKLTASNAEARLGALEARVKQNFHFDTLVRAPGSPLSQVCTRAAQ
VATFDVPVLIHGETGTGKELLARAIHYSSLRSDRPFFAVNCGAIPDELLESELFGHKKGS
FTGAHVTRIGLLDQADGGTIFLDEIGDVSPAFQVKLLRFLQEGEIRPVGSNEAKRVNVRV
LAATHRDLKGEVARARFREDLYYRLSAMVLTLPALRERRADIPVLAQSVLDALSSQHGKR
VRGFSEETLACMSAYDWPGNVRELQNEITRMLVLAERDSLSADLLSPHVVRAAAAETRAP
ETLTNSSISLPDSGPLKDRIERMEATILTETLVRCRWNKSRAAEELGLSRVGLRAKLDRY
GIARGGAFGQH