Protein Info for PS417_22690 in Pseudomonas simiae WCS417

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 TIGR01196: phosphogluconate dehydratase" amino acids 2 to 601 (600 residues), 1032.9 bits, see alignment E=0 PF00920: ILVD_EDD" amino acids 67 to 597 (531 residues), 546.3 bits, see alignment E=3.7e-168

Best Hits

Swiss-Prot: 75% identical to EDD_PSEAE: Phosphogluconate dehydratase (edd) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 99% identity to pfs:PFLU4964)

MetaCyc: 64% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U104 at UniProt or InterPro

Protein Sequence (608 amino acids)

>PS417_22690 phosphogluconate dehydratase (Pseudomonas simiae WCS417)
MHPRVLEVTERLIARSRATREAYLALIRGAAIDGPMRGKLQCANFAHGVAGCGTEDKNSL
RMMNAANVAIVSSYNDMLSAHQPYEHFPEQIKKALREVGSVGQFAGGTPAMCDGVTQGEP
GMELSLLSREVIAMSTAVALSHNMFDAALMLGICDKIVPGLMMGALRYGHLPMIFVPGGP
MPSGISNKQKADVRQRYAEGKATREELLESEMKSYHSPGTCTFYGTANTNQLLMEVMGLH
LPGASFVNPYTPLRDALTREAAHQVTRLTKANGSFMPIGEIVDEKSIVNSIIALNATGGS
TNHTLHMPAIAMSAGIILTWDDMADLSEVVPTLSHVYPNGKADINHFQAAGGMSFLIREL
LEAGLLHEDVNTVAGKGLSRYTQEPFLVDGELIWREGPIESLDETILRPVARAFSPEGGL
RVMEGNLGRGVMKVSAVAPEHQIVEAPAVVFQDQQDLADAFKAGQLEKDFVAVMRFQGPR
SNGMPELHKMTPFLGVLQDRGFKVALVTDGRMSGASGKIPAAIHVNPEAQSGGPLARVRD
GDIIRVDGVKGTLELKVDAEEFAARAPATGLLGNNVGAGRELFAFMRLAASSAEQGASAF
TSALETLK