Protein Info for GFF4432 in Variovorax sp. SCN45

Annotation: Two-component system response regulator protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 PF00072: Response_reg" amino acids 15 to 124 (110 residues), 97.5 bits, see alignment E=1.4e-31 PF00158: Sigma54_activat" amino acids 166 to 332 (167 residues), 229.8 bits, see alignment E=4e-72 PF14532: Sigma54_activ_2" amino acids 167 to 337 (171 residues), 85.4 bits, see alignment E=1.1e-27 PF07728: AAA_5" amino acids 189 to 298 (110 residues), 24.8 bits, see alignment E=5e-09 PF02954: HTH_8" amino acids 459 to 498 (40 residues), 39.1 bits, see alignment 1.2e-13

Best Hits

KEGG orthology group: K02667, two-component system, NtrC family, response regulator PilR (inferred from 86% identity to vap:Vapar_0958)

Predicted SEED Role

"Sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (516 amino acids)

>GFF4432 Two-component system response regulator protein (Variovorax sp. SCN45)
VSIPPSSSSQRPAQILVIDDEPDLRTLYELTLLREGYRVEAAGSVSEAWQHLEAGRFDAV
ITDMRLPDGQGMEIIHRIQKDQRSERCVVMTAYGSAENAVEALKAGAFDYLTKPVDLKQF
RAVVASAVQARNAAQQPAKSVPVVAAGNNDAPLVTSDSGVAALDRLVGDSEPMRLVKSRI
AKVARGMAPVLVRGESGTGKELVARAVHACSQRSDGPFVAVNCGAIPENLLEAEFFGARK
GSYTGSAQDRDGYFQAARGGTLFLDEIGDLPLAMQSKLLRAIQERSVRSIGSTQEDTVDV
RIVSATHKDLHSEVQAGRFRQDLFYRLNVIEIAVPALRDRREDLPALCAALLSRIAHDAG
MPVPHLSDDLLRRLGMHPLDGNVRELENLLHRAVALNDGDELHLDLMGGFTPSAEAAASA
PAPLMPEITTPAMLMPAPAPVAPPKPAPPPLPSDLQAYLDQQEREILVRALHDSGFNRTA
AAARLGMSLRQIRYRIARLGITTPNGDDGASSHADD