Protein Info for GFF4430 in Xanthobacter sp. DMC5

Annotation: Hydrogenase maturation factor HypD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 TIGR00075: hydrogenase expression/formation protein HypD" amino acids 2 to 369 (368 residues), 502.9 bits, see alignment E=2.4e-155 PF01924: HypD" amino acids 18 to 367 (350 residues), 506.8 bits, see alignment E=1.6e-156

Best Hits

Swiss-Prot: 66% identical to HYPD_RHOCA: Hydrogenase maturation factor HypD (hypD) from Rhodobacter capsulatus

KEGG orthology group: K04654, hydrogenase expression/formation protein HypD (inferred from 92% identity to xau:Xaut_2186)

MetaCyc: 62% identical to Fe-(CN)2CO cofactor assembly scaffold protein HypD (Escherichia coli K-12 substr. MG1655)
RXN-22646; RXN-22647

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypD" in subsystem NiFe hydrogenase maturation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (374 amino acids)

>GFF4430 Hydrogenase maturation factor HypD (Xanthobacter sp. DMC5)
MRFVDEFRDPALASALVKEIGAIAARLERGRDRPFAIMEVCGGHTHAIFRYGLEGLLPDL
VEFVHGPGCPVCVLPMGRVDDCVDIAERPEVIFTTFGDAMRVPGSKKSLLQAKADGADVR
MVYSPLDALTLAKSNPEREVVFFGLGFETTMPSTALTVQRALREGVTNFSLFCNHITIIP
TLRALLEQPDLGIDGFIGPGHVSMVIGTAPYRFIAEEFGRPLVVSGFEPIDLLQSLLMVL
RQIEAGQARIENQYARVVPEHGNPAALRAVQEVYEPRETFEWRGLGSIAGSGVRLTAPYA
SFDAERRFSVPDIKVADPAQCRCGEVLTGAAKPWACPEFGKGCTPETPLGALMVSSEGSC
AAYYQYGGMRGEAA