Protein Info for GFF4425 in Variovorax sp. SCN45

Annotation: Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.5.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 transmembrane" amino acids 23 to 50 (28 residues), see Phobius details amino acids 56 to 74 (19 residues), see Phobius details amino acids 81 to 97 (17 residues), see Phobius details amino acids 103 to 104 (2 residues), see Phobius details amino acids 106 to 127 (22 residues), see Phobius details amino acids 140 to 159 (20 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 31 to 806 (776 residues), 1094.9 bits, see alignment E=0 PF13360: PQQ_2" amino acids 236 to 325 (90 residues), 22.6 bits, see alignment E=7.6e-09 amino acids 251 to 404 (154 residues), 30.2 bits, see alignment E=3.6e-11 amino acids 683 to 783 (101 residues), 21 bits, see alignment E=2.3e-08 PF01011: PQQ" amino acids 242 to 273 (32 residues), 29.3 bits, see alignment (E = 5.1e-11) amino acids 460 to 486 (27 residues), 26.6 bits, see alignment (E = 3.7e-10) amino acids 738 to 771 (34 residues), 21.2 bits, see alignment (E = 1.9e-08)

Best Hits

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 77% identity to pfl:PFL_5668)

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (813 amino acids)

>GFF4425 Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.5.8) (Variovorax sp. SCN45)
MAQTGASGQHKGFGSMEQKRRPLAVSIGLIALGALAMLMGAFLAVGGAYLAALGGSWYFL
LAGLALLVSGLLIARHRPTGAWLFALMLAGTAMWAVRDAGWHFWPLVSRLVAFGVLAVLV
ALAYPALVRAPSAMVARGARMVAALLAVALVLVGGYAFVPTAVVVAQGPVPAVVPVKPQA
AQKDWRHWGNTTAGDRFAALDQITKDNVDQLQVAWTAHTGDVPQSNGSGAEDQNTPLQIG
TTVYVCTAYSRIVALDADTGAELWRYDPKATAPNWQRCRGLGYHDQDGLAPGATPTPGST
PGACRQRLLLPTIDARLIAVDAATGKPCTDFGKQGVVDLKAGMGEVKDGYYQQTSTPLIA
GDLVVVGGRVADNFSTDEPPGVVRTYNAVTGELAWAWDPGNPAVTRLPPPGQTYTRGTPN
VWSAMSYDPKLGLIYLPTGNATPDFWAGERTPLDDKYSSSVVALDAKSGKLRWSFQTTHH
DLWDFDLPAQPLLYDIPDGKGGTQPALAQVTKQGEIFLLDRETGKPIAEVQERPVPQGNV
PGERYSPTQPYSVGMPSIGNKTLAESDMWGATPFDQLLCRIAFKGMRHEGIYTPPGLDHA
LQFPGSLGGMNWGSVSVDPTSSYMFVNDMRLGLANYLIPRAQIPAGASGIEMGVVPQEGT
PFGAMRERFLSQLGIPCQAPPFGTMSAIDLKTRKLVWQVPVGTVRDTGPLGIRMHLPIPI
GMPTLGASLATQSGLLFFAGTQDFYLRAFDSATGQEIWKARLPVGSQSGPMTYLSPKTGR
QYVVVSAGGARQSPDRGDYVIAYALPEKKSKGK