Protein Info for PS417_22645 in Pseudomonas simiae WCS417

Annotation: C4-dicarboxylate ABC transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 PF00072: Response_reg" amino acids 5 to 114 (110 residues), 105 bits, see alignment E=6.5e-34 PF00158: Sigma54_activat" amino acids 145 to 310 (166 residues), 225.3 bits, see alignment E=9.6e-71 PF14532: Sigma54_activ_2" amino acids 145 to 315 (171 residues), 74.9 bits, see alignment E=1.9e-24 PF07728: AAA_5" amino acids 167 to 286 (120 residues), 24.5 bits, see alignment E=5.8e-09 PF02954: HTH_8" amino acids 394 to 432 (39 residues), 28.3 bits, see alignment 2.9e-10

Best Hits

Swiss-Prot: 59% identical to DCTD_PSEAE: C4-dicarboxylate transport transcriptional regulatory protein DctD (dctD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K10126, two-component system, NtrC family, C4-dicarboxylate transport response regulator DctD (inferred from 98% identity to pfs:PFLU4954)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UGS6 at UniProt or InterPro

Protein Sequence (441 amino acids)

>PS417_22645 C4-dicarboxylate ABC transporter (Pseudomonas simiae WCS417)
MLNAVIVVDDEASIRTAVEQWLSLSGFEVQLFSRAEECLAQLPKDFPGVILSDVRMPGLS
GLELLAEVQRRDADLPVILLTGHGDVPMAVEAMRDGAYDFLEKPFSPDALLNSLRRALDK
RGLILENRRLHQQADHRAQLESTLLGVSRGLQTLRRQVLDLASLPVNVLIRGETGSGKEL
VARCLHDFGPRAKKPFVALNCAAIPEQLFEAELFGHESGAFTGAQGKRIGKLEYAHGGTL
FLDEIESMPLAQQVKLLRVLQEQKLERLGSNQSIHVDLRIIAATKPDLLEEARAGRFRED
LAYRLNVAQLRLPPLRERREDIPLLFDHFAQSAAERLGRSVEPLSGAQLGRLLSHDWPGN
VRELANVAERQVLGLGEPEPEGVEAGQSLAAQQEAFEAHCLKAALTRHKGDIKAVLAELQ
LPRRTLNEKMQRHGLVREMFL