Protein Info for PS417_22640 in Pseudomonas simiae WCS417

Annotation: cytochrome C biogenesis protein CcmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 302 to 321 (20 residues), see Phobius details PF02518: HATPase_c" amino acids 473 to 580 (108 residues), 79.4 bits, see alignment E=1.4e-26

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 96% identity to pfs:PFLU4953)

Predicted SEED Role

"C4-dicarboxylate transport sensor protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U0Z6 at UniProt or InterPro

Protein Sequence (588 amino acids)

>PS417_22640 cytochrome C biogenesis protein CcmE (Pseudomonas simiae WCS417)
MIVIPRPLRLTFYSLLIIAGALLAAALATRHAERQALVDDAARANQQLALYANSLHTLIE
RYRALPAVLALDSEMINALKGPLDTATQDLLNRKLERINGAAQSSTLELMDRTGLAVAAS
NWNLPSSYVGHNYAFRPYFSQTLSQGTGRFYAVGVTTGIPGYFLSSAVVDEHEQFLGAMV
VKLEFPELEREWAQGNDLLLVSDARGIVFIANQPGWRYRNLRPLTASDLAELKATRQYDK
KHLQALETSTLQRFDENSHLMRVNGPDGSANYIWESLPLKAEGWTLHLLRKPQFAFEDQR
NAGLAAAGSWLALVFLVLFLTQRWRLARLRQRSREELEQLVEERTQALRTAQDGLVQSAK
LAALGQMSAALAHEINQPLTAQRMQLATLRLLLDHGRVDDAYQALTPLDDMLTRMAALTG
HLKTFARKSPSGLRERLDLATVVDQSLHLLDARLRDESIGVVLDLTRPAWVRGDAIRLEQ
VLINLLRNALDAMVDKPRKRLEIRLHADEQLWQLTVSDSGGGIAEEHLNSVFDPFFTTKP
VGDGLGLGLAVSYAIVHELGGRLIASNRGDGAVFTLTLPIALETPDLC