Protein Info for PS417_22575 in Pseudomonas simiae WCS417

Annotation: exonuclease I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 PF00929: RNase_T" amino acids 6 to 185 (180 residues), 88.9 bits, see alignment E=5.6e-29 PF08411: Exonuc_X-T_C" amino acids 204 to 467 (264 residues), 332.7 bits, see alignment E=1.4e-103

Best Hits

KEGG orthology group: K01141, exodeoxyribonuclease I [EC: 3.1.11.1] (inferred from 95% identity to pfs:PFLU4940)

Predicted SEED Role

"Exodeoxyribonuclease I (EC 3.1.11.1)" in subsystem DNA Repair Base Excision (EC 3.1.11.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U5Y9 at UniProt or InterPro

Protein Sequence (475 amino acids)

>PS417_22575 exonuclease I (Pseudomonas simiae WCS417)
MTSIFWYDYETTGINPRSDRPLQVAGIRTDLELNEIGEPVNLYCQPSDDILPHPVACAIT
GITPAILAEKGLAEADFMTRVHAQLAAPGTCGAGYNTLRFDDEMTRYSLYRNFFDPYARE
WQGGNSRWDLIDVIRTAYALRPEGIEWPQQDGRVTLKLERLTEANGIDHGQAHDALSDVR
ATIALARRVREKQPKLYEWLFQLRSKQRVMDQVRLLQPMVHISGRFSAERHYLGVVLPLA
WHPRNRNALIVCDLGLDPQGLLDLDADTLRQRLYTRREDLAEGELPVPLKLVYINRCPVV
APLNVLRTEDRERLHLDMNIYQERALRLTDAQEVWRDKLAAVYAEEGFASSSDPEQQLYD
GFIGDRDRRLCEQVRTAEPAQLARQQWPFDDHRLPELLFRYRARNFPDTLNSEEQQRWQL
FCQQRLSDPEWGAPNTLAAFKQAKQEFAVSATPFQTEVLERWQEYADSLAHKVNL