Protein Info for GFF4408 in Xanthobacter sp. DMC5

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16078: 2-oxogl_dehyd_N
  • amino acids 13 to 52 (40 residues), 63.6 bits, see alignment (E = 2e-21)
• TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component
  • amino acids 15 to 982 (968 residues), 1167.7 bits, E=0
• PF00676: E1_dh
  • amino acids 243 to 559 (317 residues), 178.5 bits, E=3.2e-56
• PF02779: Transket_pyr
  • amino acids 629 to 822 (194 residues), 170.9 bits, E=4.2e-54
• PF16870: OxoGdeHyase_C
  • amino acids 827 to 983 (157 residues), 180.4 bits, E=3.9e-57

Best Hits

Swiss-Prot: 73% identical to ODO1_OCHA4: 2-oxoglutarate dehydrogenase E1 component (sucA) from Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 88% identity to azc:AZC_4013)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.2

Protein Sequence (984 amino acids)

>GFF4408 2-oxoglutarate dehydrogenase E1 component (Xanthobacter sp. DMC5)
MSRAEQNDVFLNTSFLYGANAAWIEDLYARYETDPNSVSAEWQAFFAALKDDPNQVIENA
RGASWKKPNWPVHANGELVSAMDGQWIEVEKAIADKVKAKAQKSGVEFSATEVQQATRDS
VKALMMIRAYRMRGHLHANLDPLGLTPPREAPELDPASYGFYEADLDRKIFIDHVLGLEF
ATVREMVAILRRTYCASVGVEFMHISSPEEKAWIQERIEGPDKEVTFTREGKRAILNKLV
EAEGFEKFLDVKFTGTKRFGLDGGESLIPALEQIIKRGGNLGLKEIVFGMAHRGRLNVLT
QVMGKPHRALFHEFKGGSWAPDDVEGSGDVKYHLGASSDREFDNNKVHLSLTANPSHLEI
VDPVVLGKARAKQDQLGDTERTKVLPLLLHGDAAFAGQGVVAECLGLSGLKGHRTGGSIH
FIINNQIGFTTNPRYSRSSPYPSDVAKMIEAPIFHCNGDDPESVVFCAKVATEFRQRFHK
PVVVDMFCYRRFGHNEGDEPAFTQPKMYKVIRQHPSVLELYTKKLESEGVVTAGEVDTMR
NAWRDRLEAEHEAGQAYKPNKADWLDGRWAGFKPTHEDDDPRRGNTGLPEDELKAIGAKI
TTVPEGFQVHRTIQRFLDNRKKAIEDGKGIDWATGEALAFSSLLLEGHPVRLSGQDVERG
TFSQRHSVLLDQETEARYKPFNHLSETQAKYEVINSMLSEEAVLGFEYGYTLSEPNTLVL
WEAQFGDFANGAQVVFDQFISSGERKWLRMSGLVCLLPHGYEGQGPEHSSARLERYLQLC
AEDNMQVANVTTPANYFHILRRQMKREFRKPLILMTPKSLLRHKRAVSNLSEMVTGTTFH
RVLWDDAQSHPDESAIKLQPDDKIRRVVLSSGKVYYDLLEEREKRGVDDVYLMRVEQLYP
FPLKTLVQELGRFKNAEVVWCQEEPKNQGSWAFVQPYLEWVLEQAGGTARRPRYAGRPAS
AATATGLMSKHMAQLKAFLEEALG