Protein Info for GFF4406 in Variovorax sp. SCN45

Annotation: Alkane-1 monooxygenase (EC 1.14.15.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 753 transmembrane" amino acids 207 to 224 (18 residues), see Phobius details amino acids 361 to 380 (20 residues), see Phobius details amino acids 386 to 404 (19 residues), see Phobius details amino acids 416 to 438 (23 residues), see Phobius details amino acids 469 to 491 (23 residues), see Phobius details amino acids 506 to 526 (21 residues), see Phobius details amino acids 546 to 564 (19 residues), see Phobius details amino acids 586 to 609 (24 residues), see Phobius details amino acids 683 to 702 (20 residues), see Phobius details PF00111: Fer2" amino acids 14 to 86 (73 residues), 65.3 bits, see alignment E=7.8e-22 PF00970: FAD_binding_6" amino acids 108 to 200 (93 residues), 47.6 bits, see alignment E=3.5e-16 PF00175: NAD_binding_1" amino acids 210 to 311 (102 residues), 75.3 bits, see alignment E=1.2e-24 PF00487: FA_desaturase" amino acids 470 to 679 (210 residues), 99.2 bits, see alignment E=6.8e-32

Best Hits

KEGG orthology group: None (inferred from 80% identity to pna:Pnap_4185)

Predicted SEED Role

"Alkane-1 monooxygenase (EC 1.14.15.3)" (EC 1.14.15.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.15.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (753 amino acids)

>GFF4406 Alkane-1 monooxygenase (EC 1.14.15.3) (Variovorax sp. SCN45)
MNFLHGRSLHQAEINGQTITVEPDETLLEAAQRQNINIPSICRVGGCGTCKCKLKSGKVD
ELTETAYLLTEKEISDGFILACQSRLRSDVKIELDREGAIDGPPVKGVIVGKQMLTHDIA
RIDLRLDTPIRYRAGQFAEVTLSSMADAPRSYSFSTAPGNDRQVSFTVRRVPGGRVSGHL
VDQAQVGESVTVRGPGGHFYMRPGREPVLMVAGGSGLAPILAMLQDMKRSGDMRPVTVLF
GARSQKDLYALDEMAAYEKAWPNFRFVPVLSAADPSSPWDGRTGLVTEHIPELLGGATSA
YLCGPPAMIDSAIAALRQRGIADTNLHADRFVDQAPKLHTGFYGAVGLPTVERVPANILD
YLKFFLLHGVGIGAVASILAGGVYTSLGLLGFLIFYIGGDALLGHDTRTPKYKSPWILTV
QLWFILPLVALIVFAYVWTLSSGDPLGFGAWVTHLTQYDILAAREATGFWHHLAGGVFTG
FMIGVLGTITAHELTHRTWEPVSMFIGRWLLSFSYDVGFAIEHVYGHHRYVSTEHDPATA
PRGRNVYLHIVMSTIRGNVSAWRIEAERLKRKHLPVISIHNAYIRGLLMSAVLVAIAYWM
AGWIGVLGFTGSALFGKSLLEIVNYMEHYGMVRAPDQAVQLRHSWNTNARMSSWAMFNLS
RHSHHHAQGEVPYQNLMPLPEAPTMVAGYLSTILLTLLPPLWQRIMVPKIVEWDRHYATP
EERVLALRANQKSGLKALRRYDPRQWNKELAAD