Protein Info for PS417_22525 in Pseudomonas simiae WCS417
Annotation: ATP--cobalamin adenosyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to ATR_CUPMC: Cobalamin adenosyltransferase (cobO) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)
KEGG orthology group: K00798, cob(I)alamin adenosyltransferase [EC: 2.5.1.17] (inferred from 95% identity to pfs:PFLU4930)Predicted SEED Role
"ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17)" (EC 2.5.1.17)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (28/36 steps found)
- adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
- adenosylcobalamin salvage from cobalamin (5/5 steps found)
- adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
- adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
- cobalamin salvage (eukaryotic) (4/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.17
Use Curated BLAST to search for 2.5.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UK52 at UniProt or InterPro
Protein Sequence (192 amino acids)
>PS417_22525 ATP--cobalamin adenosyltransferase (Pseudomonas simiae WCS417) MGFRLSKIYTRTGDKGETGLGDGRRVPKDHPRVEAIGEVDTLNSQLGLLLASLEEQSGKH PELKDVIEVLTPCQHRLFDLGGELAMPVYKALNAAEVDRLEAAIDRWNEDVGPLENFILP GGSALIAQAHVCRSLARSAERRCQQLNAVEPLEGVGLAYINRLSDLLFVAARVIAKRQGV AEVLWQAAAKPH