Protein Info for GFF439 in Variovorax sp. SCN45

Annotation: Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 signal peptide" amino acids 1 to 6 (6 residues), see Phobius details transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 33 to 52 (20 residues), see Phobius details amino acids 64 to 84 (21 residues), see Phobius details amino acids 90 to 111 (22 residues), see Phobius details amino acids 171 to 192 (22 residues), see Phobius details amino acids 223 to 243 (21 residues), see Phobius details amino acids 263 to 280 (18 residues), see Phobius details amino acids 300 to 322 (23 residues), see Phobius details TIGR00380: cobalamin biosynthesis protein CobD" amino acids 14 to 306 (293 residues), 205.6 bits, see alignment E=5.4e-65 PF03186: CobD_Cbib" amino acids 17 to 294 (278 residues), 266.8 bits, see alignment E=1.1e-83

Best Hits

KEGG orthology group: K02227, adenosylcobinamide-phosphate synthase CobD [EC: 6.3.1.10] (inferred from 76% identity to vap:Vapar_2555)

Predicted SEED Role

"Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)" (EC 6.3.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.10

Use Curated BLAST to search for 6.3.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (325 amino acids)

>GFF439 Adenosylcobinamide-phosphate synthase (EC 6.3.1.10) (Variovorax sp. SCN45)
MPADDPLSYSFALVAALWLALAIDRWLGEPRPYLHPVVWMGNYLGWIGSRAAPLHHHAKP
VDMPAFFIGALTWCVGAMAVWGVASGLQGAIVQWLPAWAVAVLLGLLFKPLFAWRMLRDE
VLAVEDALAVSLDAGRAQLARLVSRNVAELGEREVRESAIESLAENLNDSLVAPVFWFVL
FGLPGAAIYRFANTADAMWGYRGERGGRDWTWFGKWAARADDVLSWVPARITAVLLAVAA
LRWPARLSEEARRTPSPNSGWPMAAMALLLGVRLAKPGVYALNAEGRAPASADTRRAAQL
GAVVVLAWAVCASLVIVAVLGGTGG