Protein Info for GFF4377 in Xanthobacter sp. DMC5

Annotation: Anthranilate 3-monooxygenase oxygenase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 PF11794: HpaB_N" amino acids 4 to 270 (267 residues), 303.2 bits, see alignment E=1.9e-94 PF03241: HpaB" amino acids 278 to 476 (199 residues), 191.1 bits, see alignment E=1.3e-60

Best Hits

KEGG orthology group: K00483, 4-hydroxyphenylacetate-3-hydroxylase large chain [EC: 1.14.13.3] (inferred from 90% identity to xau:Xaut_0377)

Predicted SEED Role

"4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic Amin Catabolism (EC 1.14.13.3)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.3

Use Curated BLAST to search for 1.14.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>GFF4377 Anthranilate 3-monooxygenase oxygenase component (Xanthobacter sp. DMC5)
MIKTGAEHIESLKDGREIFIEGQRVGDATTHHAFRRSIASVGKMFDFHSAPENRELMTFE
TDTGTRANRIWQLPTSYEDLKTRRRGLEAWTELHGGFLGRAPDHVASCISGMYMGLDQFE
LYDPARARALADYYRYARDNDLYLTYVIINPQADRSKNAAEQADPFLSAAVVDEDTSGIT
VRGAKMLATGGIMANEVFVTCIQPLQKGEEPYALSFCIPMNAKGLKILSRKSYEAGAPSV
FDNPLSSRFDENDAVLYFDDVKVPWDRIFIARDIAMTNKQFHATPAHVYQNYQAQIRLSV
KLKFLLGIARRITEVNGTTNFPQVRETLGQLAAEAGMVDAFVAAMEAKGSYYGPYFVPDR
HTLYAAQTLAQQLYGKFITTLRELAGGGMIMLPSSVHDYENAELVRLIGKTQQSPAASAE
EKVKFYKLAWDAVGSEFASRHTQYEMFYAGASFVVKGHSFRTYDWAGAEKLLASMLSSYD
LADEVTPRVARAAE