Protein Info for GFF4371 in Sphingobium sp. HT1-2

Annotation: Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 PF00078: RVT_1" amino acids 89 to 235 (147 residues), 86.3 bits, see alignment E=3.7e-28 PF01348: Intron_maturas2" amino acids 396 to 490 (95 residues), 43.1 bits, see alignment E=6.6e-15 PF21368: AI2M-like_HNH" amino acids 550 to 596 (47 residues), 57.6 bits, see alignment 1.4e-19

Best Hits

KEGG orthology group: None (inferred from 50% identity to bpt:Bpet4285)

Predicted SEED Role

"Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)" (EC 2.7.7.49)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (618 amino acids)

>GFF4371 Retron-type RNA-directed DNA polymerase (EC 2.7.7.49) (Sphingobium sp. HT1-2)
MLPDTVYKRLGTIGNLTRQGKRINGLSRLMQSRILWEKAYAEMASNKGALTRGVTNNTLD
GFSFERVENLIARISAGDYRFTPVRRVYIPKANGKKRPLGVPTADDKLVQGVVKLLLEVV
YEPVFSVHSHGFRRGRSCHTALDKIKNGWTGVKWFVDVDVVGFFDNIDHRILLNLLKKRI
DDKRFIKVVEGMLKAGYMEEWKFHATMSGTPQGGIVSPILANIYLHELDEFLEQEKARFD
KGRMRAFTRRYRTLATAIRLNRQKADVLYAEGRTVEADRLKQKVAKLEAERRSTPSKDGF
DPNYRRLLFCRYADDFLIGVIGSKADARDIMQRVTDFLRDELRLEASAEKSGISKATTGT
LFLGYGVKTATANRLRKTRIGGRVVLMRSPGDRVRLLVPQNRIVRFNREKGYGDLGRLRA
VHRAYLIDSSALEIVLAYNAEMRGFANYYRLAYFVKHSLNKLYFLWRTSLLRTLASKYRS
SVNKVVHQLRSGTDLAVSVKMGGKERGASVFKMKEIDQLPKLGQAVDIRPVVQFTKARSD
VLDRLRANACEYCGRQDQPCEVHHVRRMRDMKGSELWQQVAAARRRKRVVLCRPCHRALH
AGKLTRQTVIDTQDWRAG