Protein Info for GFF435 in Xanthobacter sp. DMC5
Annotation: 15-cis-phytoene synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 51% identical to CRTB_PANAN: 15-cis-phytoene synthase (crtB) from Pantoea ananas
KEGG orthology group: K02291, phytoene synthase [EC: 2.5.1.32] (inferred from 81% identity to xau:Xaut_3578)Predicted SEED Role
"Phytoene synthase (EC 2.5.1.32)" in subsystem Carotenoids (EC 2.5.1.32)
MetaCyc Pathways
- neurosporene biosynthesis (2/5 steps found)
- β-carotene biosynthesis (engineered) (4/8 steps found)
- trans-lycopene biosynthesis I (2/6 steps found)
- trans-lycopene biosynthesis II (oxygenic phototrophs and green sulfur bacteria) (3/8 steps found)
- lycopadiene biosynthesis (1/7 steps found)
- superpathway of carotenoid biosynthesis in plants (7/22 steps found)
KEGG Metabolic Maps
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.32
Use Curated BLAST to search for 2.5.1.32
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (313 amino acids)
>GFF435 15-cis-phytoene synthase (Xanthobacter sp. DMC5) MSRTDAAPDVVGASEEAIAKGSKSFAAAARLFAPRMREDAVMLYAWCRHCDDVVDGQELG HGRIASAQTPAERLDALFEETRRAYRGVPSSHPAFAAFAEVVRRNDIPERHPLDLLEGFR MDVEDRRYETLDDTLTYCYHVAGVVGVMMALIMGARDEVVLDRASDLGLGFQLTNISRDV IEDAGIGRIYVPETFLLMEGVPDGGIAAPEHRPQVAAAVARLLDVAEPYYEQAVIGMGAL PFRAAAAVGAARCVYRGIGTEVRRRGPKAWNARVSTTKAQKIGYLMQGIGLALATRRSGP QPPRPTHLWTRPR