Protein Info for GFF4340 in Sphingobium sp. HT1-2
Annotation: Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 40% identical to HEMN_BRADU: Oxygen-independent coproporphyrinogen III oxidase (hemN) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 66% identity to npp:PP1Y_AT1772)Predicted SEED Role
"Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.99.22)
MetaCyc Pathways
- heme b biosynthesis II (oxygen-independent) (3/4 steps found)
- superpathway of heme b biosynthesis from uroporphyrinogen-III (4/6 steps found)
- 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (2/9 steps found)
- superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.22
Use Curated BLAST to search for 1.3.99.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (452 amino acids)
>GFF4340 Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22) (Sphingobium sp. HT1-2) MLLNETGCLAQQDRTMWPYHPDLLATPVPRYTSYPTAAEFGNDVGEQDFASALSRTDGDV SLYVHIPFCETICWYCGCNTGRANRRQRLASYLEALHSEIALVGRMLPKTATVRRLSFGG GSPNAITATDFVRLFLSLTDSFRLEKPVVSIELDPRTLTSAWAQAIAGTRISNASLGVQT FDPRLQEAIGRIQPLQDIERCTSMLREAGVTSLNYDLMYGLPGQSEKDLADSLAKAVELG ADRIALFGYAHVPHLIARQRRIDADALPDQAMRFRMASQGHELLTRSGYEAVGFDHFALP HDGLAQATRESRLSRNFQGFTDDDAPVVIGLGASAISSFPDLLIQNEKNAGRYRMMLSQQ RLTGNRGIRRNADDRLRGGVIEQLLCQGKASVPADLLLPAHDRLAPFLDRGLVCWEGDTL SIAPSALPYARTIAATFDAYRQPGSRRFSSAV