Protein Info for GFF4325 in Xanthobacter sp. DMC5

Annotation: Cadmium, zinc and cobalt-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 627 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 43 to 63 (21 residues), see Phobius details amino acids 75 to 100 (26 residues), see Phobius details amino acids 129 to 147 (19 residues), see Phobius details amino acids 240 to 259 (20 residues), see Phobius details amino acids 265 to 288 (24 residues), see Phobius details amino acids 575 to 594 (20 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 74 to 617 (544 residues), 470.6 bits, see alignment E=1.3e-144 TIGR01494: HAD ATPase, P-type, family IC" amino acids 98 to 336 (239 residues), 103.4 bits, see alignment E=1.5e-33 amino acids 423 to 593 (171 residues), 95.5 bits, see alignment E=3.8e-31 PF00122: E1-E2_ATPase" amino acids 125 to 295 (171 residues), 135.8 bits, see alignment E=1.2e-43 PF00702: Hydrolase" amino acids 311 to 528 (218 residues), 79.1 bits, see alignment E=6e-26

Best Hits

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (627 amino acids)

>GFF4325 Cadmium, zinc and cobalt-transporting ATPase (Xanthobacter sp. DMC5)
MSEPGQIQSEAAAGRNRYLLLAAAVLPLAVGLLLQLMGMKAAASAAFAVGTAPVLAVLLL
DIARSLRAGRFGLDVLAALSMAAALAFGEPLAGNVVALMYAGGQQLERFAEGRARREMTA
LAARAPRDAVLHLDGGLVIVAAVDLVAGDRVLVRSGDTVPADGAVIGGPAVLDLSSLTGE
TRPARLEDGEEAPSGAVNAGDAFDLAIVRPAAESTYAGIVRLVEEAASSRAPMVRLADRY
ALAFLLISLALAIGAAVLAHDPRRALAVLVVATPCPLILAVPVALMAGLSRAAKRGVLVK
SGGALEKLARVRALVVDKTGTLTHGRAELEEVRTAPEFEPDDVLRLAASLDQASSHVVAQ
ALVGAAQGSGLALSPPLGVKEDGGAGLEGWVEGRAVAVGGTAYVAARASGDWSELRAQLP
PASAVVAVAVDGRPAGLLLFADPVRDDAPEMIAALRRGGVAYVALASGDRSEAVDAVGLA
LGLDAWTGELTPQGKIEHIALARGTVKGGGVMMVGDGVNDAPALAAADIGVALGARGAAA
SAEVADAVLLVDRLDRLAEAMAIARRSLAIARQSVRWGIGLSLVGMVAAAFGYLQPVQGA
LLQEAIDVVVILNALRVLIPGPGEGAD