Protein Info for GFF4306 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02743: type-F conjugative transfer system protein TraW" amino acids 17 to 212 (196 residues), 198.5 bits, see alignment E=5.2e-63

Best Hits

KEGG orthology group: K12061, conjugal transfer pilus assembly protein TraW (inferred from 51% identity to nar:Saro_4002)

Predicted SEED Role

"IncF plasmid conjugative transfer pilus assembly protein TraW" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (219 amino acids)

>GFF4306 hypothetical protein (Sphingobium sp. HT1-2)
VIRQVALAGGFLAIALVASSAWPVVEGALAHDHGVMGQTWGIVEPDLLDTIDRRLKTMQA
DGSIERMQAALRVKAQERVRNPLPVAGIGAVREARSWTFDPSIVLDQDVRDQKGRLVAQA
GQRVNPLSFVAMKTDLVFIDGRDDAQVEWAMKRWSAQNAKIIFVAGSPFDRMGEKKRRFF
FDQQGKLTSHFGIAHVPAVVAPQGEVLRISEIEMPGAGA