Protein Info for GFF4304 in Sphingobium sp. HT1-2

Annotation: IncF plasmid conjugative transfer pilus assembly protein TraU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 337 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF06834: TraU" amino acids 31 to 336 (306 residues), 404.5 bits, see alignment E=1.6e-125

Best Hits

KEGG orthology group: K12060, conjugal transfer pilus assembly protein TraU (inferred from 72% identity to nar:Saro_4000)

Predicted SEED Role

"IncF plasmid conjugative transfer pilus assembly protein TraU" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (337 amino acids)

>GFF4304 IncF plasmid conjugative transfer pilus assembly protein TraU (Sphingobium sp. HT1-2)
MMRRLVGLLAFLAALAAWSAPASALPANCTGKFVNPVTDVCWSCLFPLSIGGAHIWPGSR
PDTKNPSLPVCACGSPVPRIGISIGFWEPARLVDVTTKPWCFPNLGGLRLNPGLDIGQGQ
YTGPRIGGGTTQNSANWQAHYYVYPLLYWMEILTDFLCFEQASFDVAYMTEIDPLWNDDV
LTTLINPEVALFNNPIAVAACAADCAAATAALPIDQMFWCSGCNGSMFPMNGNIAAHNSP
VQSSRLAAERLLFKMHRQGLAWGTAGSKALCNKYVMPILKKSQYRIQMTNPTPTVSGKFA
CSTIGASTLPPDAGRAFPVGGEDFGYLLWRKRNCCMF