Protein Info for GFF4302 in Sphingobium sp. HT1-2

Annotation: IncF plasmid conjugative transfer protein TraN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 697 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 546 to 569 (24 residues), see Phobius details PF06986: F_T4SS_TraN" amino acids 378 to 646 (269 residues), 271.2 bits, see alignment E=4.2e-85

Best Hits

Predicted SEED Role

"IncF plasmid conjugative transfer protein TraN" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (697 amino acids)

>GFF4302 IncF plasmid conjugative transfer protein TraN (Sphingobium sp. HT1-2)
MAGRGILASAVLALLSLPGAALAQTSGQASPSGGYYDLQDYPYFNEAAQPTPPAQASPGP
SATSAPQPAPAQSAMSAPSLTVTQAPSASSAAARVQAQAEQQAPTQTPGQPYVYPKGDLA
AAREQAKALGKSSRASAASLPTSTPLSQVPGYSATANPAQAYADDPDALIAAGGSAAGHN
DAWRMVTDPGRLKVTLGAGEVGRAQDVEKDPNSYLSGQSLGAADGSCKPLPPGGSAGYYE
ATCNTGTKVEESAAVCRTPLVMDVTPGSTKYIYVCENWIGNPPRAGNNTGRRCFPAFTQP
VANGVCRVRSRETVHYPICHQGTLGKCFEPDVADGEEITYECDSPAVARSYVVETTGQVV
NERRDETMCNASTAGKTCELKSETCVDPDPTTRTVNGVQITRACWNWERTYSCVGTTQAS
DCGELAGNPQCVYVRDECLDDPQVGPCQVTTKVYKCPIPTAPTTQPKQYICGDDVYCLDG
QCEPVEREASTEFKDAVVGLHALGQAKEEFNPDTMTLFSGKRQTCHKPVFGLVNCCAGKA
SGLITTAAGAAAIAGGPAAIAALATPFLTMFMCSSQEKLLDVQDRMGMCHKLGQYCTDKV
LGICTSRSTAYCCFESKLSRILQEQGRPQINKPWGSAKKETCKGFTLDEFSRLDLSKMDF
TEVYAEFVDAAKLPDEIETSRQIQERIKAYYDSKAGK