Protein Info for GFF4271 in Xanthobacter sp. DMC5
Annotation: Acetyl-coenzyme A synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to Y3568_PSEAE: Uncharacterized protein PA3568 (PA3568) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 90% identity to xau:Xaut_2464)Predicted SEED Role
"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)
MetaCyc Pathways
- acrylate degradation II (3/3 steps found)
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- superpathway of acetate utilization and formation (3/3 steps found)
- superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
- acetate and ATP formation from acetyl-CoA III (1/1 steps found)
- acetate conversion to acetyl-CoA (1/1 steps found)
- chitin deacetylation (3/4 steps found)
- L-isoleucine biosynthesis V (2/3 steps found)
- ethanol degradation III (2/3 steps found)
- β-alanine biosynthesis II (4/6 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (6/9 steps found)
- 2-methylcitrate cycle I (3/5 steps found)
- 2-methylcitrate cycle II (3/6 steps found)
- adlupulone and adhumulone biosynthesis (1/6 steps found)
- colupulone and cohumulone biosynthesis (1/6 steps found)
- lupulone and humulone biosynthesis (1/6 steps found)
- cis-geranyl-CoA degradation (1/9 steps found)
- superpathway of bitter acids biosynthesis (3/18 steps found)
KEGG Metabolic Maps
- Glycolysis / Gluconeogenesis
- Propanoate metabolism
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.1
Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (638 amino acids)
>GFF4271 Acetyl-coenzyme A synthetase (Xanthobacter sp. DMC5) MSIDGASRYAQAYAGWKTDPSAFWAEAAQGIDWARPPQAIFDADAGVYGRWFPDAVGNVC HNALDRHVAAGRGDQAALIYDSPVTGTKRTLTYGEMLKETATLAAVLQDMGVGKGDRVLV YMPMVPEAICAMLACARIGAIHSVVFGGFAAKELATRIEDAEPKVILAASCGIEPNRVVP YKPLLDLAISMSAVKPERCLVLQRSQGPGALVEGRDLDWQAAVDAAASAGKAAACADMLA TDPLYILYTSGTTGKPKGVVRDTGGYMVALDWSMKNLYGIKPGEVFWTASDIGWVVGHSY IVYAPLILGATTLVYEGKPVGTPDAGAFWRVIEEHGVVALFTAPTALRAIKKEDPDCLLK QGRDLSRFRTLFLAGERADPDTVVWAQEQLKVPVVDHWWQTETGWAIAANPVGLGLLPIK LGSPTVPMPGYDVQIVDEASKPVPAGTMGSIVVKLPLPPGCLPTLWQQDDRFAESYLAEF PGYYKTADAGFLDDDGYVFVMGRTDDIINVAGHRLSTGGMEEVLASHPDVAECAVIGVKD ELKGEVPCGFVVLKAGERRAPDVIEKELVGLVRDRIGPVAAFKLAVTVNRLPKTRSGKIL RGTMKKIADHDPWTVPATIDDAGVLEEIEGVLKERGIA