Protein Info for GFF4264 in Xanthobacter sp. DMC5

Annotation: Phosphoglycolate phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 PF00702: Hydrolase" amino acids 6 to 197 (192 residues), 73.3 bits, see alignment E=7.1e-24 PF12710: HAD" amino acids 8 to 194 (187 residues), 32.3 bits, see alignment E=2.7e-11 PF13419: HAD_2" amino acids 78 to 203 (126 residues), 69 bits, see alignment E=1.2e-22 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 118 to 197 (80 residues), 24.8 bits, see alignment E=1.2e-09 PF13242: Hydrolase_like" amino acids 159 to 227 (69 residues), 49.6 bits, see alignment E=6e-17

Best Hits

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 76% identity to xau:Xaut_2473)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (247 amino acids)

>GFF4264 Phosphoglycolate phosphatase (Xanthobacter sp. DMC5)
MQDLTALLFDKDGTLVHFDRTWGRAAGAVMRQMARDDAHAVARLEAVSHYLPDEERFLPT
SPLVSGSSAHYGPLWAHALGRPADGDFLAEIDRLFAAEGLANLTPIGHPARALQRLKAAG
FALGIVTNDAEENARQQAEALGFLDLLDAVHGYDSGFGSKPGPGMVAAFAARFGHCPDAM
AVIGDSAHDLASARGAGARFILVRSGPAPVDDLVTDADLVVDSVDDLPDVLLASGGKASS
LAGENAV