Protein Info for PS417_21835 in Pseudomonas simiae WCS417

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 transmembrane" amino acids 20 to 46 (27 residues), see Phobius details amino acids 83 to 104 (22 residues), see Phobius details amino acids 116 to 137 (22 residues), see Phobius details amino acids 147 to 170 (24 residues), see Phobius details PF05230: MASE2" amino acids 14 to 101 (88 residues), 84.1 bits, see alignment E=5.6e-28 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 182 to 344 (163 residues), 168.6 bits, see alignment E=5e-54 PF00990: GGDEF" amino acids 186 to 340 (155 residues), 148.3 bits, see alignment E=1.7e-47

Best Hits

Swiss-Prot: 38% identical to DGCC_ECOL6: Probable diguanylate cyclase DgcC (dgcC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 84% identity to pfs:PFLU4782)

MetaCyc: 38% identical to diguanylate cyclase DgcC (Escherichia coli K-12 substr. MG1655)
Diguanylate kinase. [EC: 2.7.7.65]

Predicted SEED Role

"Response regulator protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.65

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U7X1 at UniProt or InterPro

Protein Sequence (349 amino acids)

>PS417_21835 histidine kinase (Pseudomonas simiae WCS417)
MENRRGKGLSFARRIYKPRIIGLGIGCISVTGALYPLAMPGWVWAFLLFNGFAWAHVAYQ
LSARSQFPYQAEQRNLLYDSLFGGFWAAATQFTPLTTVTILSMMTMNNVAAGGKRLFLRG
LLAQVAGIGVASAFFGIKFNPNVSLTQVYTCLPMLTLYPMAIGMVCYELAIKLSEHKRAL
SALSRTDSLTGLLNHGSWKDLLHLKFHKCQQQQSHATIALIDIDHFKQINDTHGHIVGDA
VLRQLSLELRRNLRENDLAGRYGGDEFCVILPQMPMEQAAQVMERMRETFSNYRNPQIPE
LRVSLSIGLADFQPVFTDAAMWLNAADRALYAAKDTGRNRVNMSDERVA