Protein Info for GFF4256 in Variovorax sp. SCN45

Annotation: Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 7 to 90 (84 residues), 72.8 bits, E=1.8e-24
• PF19112: VanA_C
  • amino acids 143 to 332 (190 residues), 134 bits, E=7.5e-43

Best Hits

KEGG orthology group: K03862, vanillate monooxygenase [EC: 1.14.13.82] (inferred from 58% identity to vap:Vapar_6309)

Predicted SEED Role

"Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82)" in subsystem Phenylpropanoid compound degradation (EC 1.14.13.82)

MetaCyc Pathways

• vanillin and vanillate degradation II (2/2 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.82

Use Curated BLAST to search for 1.14.13.82

Protein Sequence (357 amino acids)

>GFF4256 Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82) (Variovorax sp. SCN45)
MFVKNCWYVAAWDHEVLADTLLARTILEQPVLLYRRADGVAVAIDNRCAHRGAPLSMGRR
EGDAIRCMYHGLKFGADGRCTEVPGQDNVPARLCVRSYPVAERGRWIWIWMGDPQRADVA
LIPDAFSLKHPDWRYKPGYLHYDTDYLLICDNLLDFSHLSYVHERTLGGSPNIAQSRPVV
TPLARGLNVTRDVRGTVPAPYHARLGGFQGKVDRRFNYDFLLPGVLLMHAHVKPSDTPDD
DMSGALQFHSCQALTPETAHSTHYFFMQAHRFRLDDATITESIYQSLCTAFEEDRQIIMA
QSRMLAATPGGQMHPIAADLALARFRRLVQEFSEGERGGEATPLPMAIHRPVHAPIA