Protein Info for GFF425 in Xanthobacter sp. DMC5

Annotation: Oxygen sensor protein DosP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 830 PF13426: PAS_9" amino acids 20 to 88 (69 residues), 16.6 bits, see alignment E=2.8e-06 amino acids 126 to 228 (103 residues), 34.8 bits, see alignment E=5.8e-12 TIGR00229: PAS domain S-box protein" amino acids 112 to 230 (119 residues), 23 bits, see alignment E=7e-09 PF13188: PAS_8" amino acids 116 to 150 (35 residues), 20.9 bits, see alignment (E = 9.5e-08) PF08448: PAS_4" amino acids 123 to 231 (109 residues), 32.1 bits, see alignment E=4.2e-11 PF13185: GAF_2" amino acids 249 to 391 (143 residues), 41.6 bits, see alignment E=5.1e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 400 to 561 (162 residues), 153.2 bits, see alignment E=5.1e-49 PF00990: GGDEF" amino acids 403 to 558 (156 residues), 163.6 bits, see alignment E=1.2e-51 PF00563: EAL" amino acids 580 to 814 (235 residues), 236.4 bits, see alignment E=1.1e-73

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (830 amino acids)

>GFF425 Oxygen sensor protein DosP (Xanthobacter sp. DMC5)
MDGNMADRPAAARAQGREAVVFFDTNNLVTFINTAAERLWGLNEGEAIGRPVGDLIPGAG
GQPSGELPLLITRDDGSRVEGLVSVTRAKHKRETYYIAFVRPTLNPPVHTKSLAILAAVA
EKTDRGVLVADAHGKVIYANPAFRDMLDDGTEWPESVTLAELFCGRLGNACFADHIQDAM
DRRIALSEDVALKGNTGMEVWVSASVTPIMDRQTGVLEQVVAVVADITHTRLVEDLQRQV
LGALAADAPMADIMDILCRKVQGLAPNALCSILGVTADDRLTVLAAPSLPAEFSAAVEGL
PIGPRSGSCGTAAFRGTPVLVEDIATSPLWEDFRDIPIPPGITGCWSIPVKLRGGKVAAT
FAFYFQTAHGPTPWLENVVAACSDLCALALERFEAKKRIEMLANVDTLTGLANRRQLHAV
LEELLVEARTLEQPLAVLYLDIDHFKDVNETLGHSTGDQLLVEVSRRLKAQLRTGDVVGR
TGGDEFLVLVHDCTAEEAARIARRLLEYVAAPITAQALHLTMSASVGIALYPDHGTDAET
LLKHADTAVHEAKRKGRGKFHFFTEELNRRVLDRLVLSVALREAIQNRALRLVYQPQVLL
SSGALHGVEALARWTDPIHGDVPATRFIPLAEEYGLIESIDEWAVEASCQRIATWRQEGV
DVPHVSVNLSPLSFRSGDIVGFISGALREYDLKPSDLRVEITERVMMDQHPNSFATARAI
EALGVRIAMDDFGTGYSSLSALSRLPIAELKIDRSFMLSIEQDQNAQALATAVIRIGHSL
GMTVVAEGVETQAQADLLRFLGCHAAQGFLFARPIEAADIGSWLAARRAG