Protein Info for GFF4245 in Xanthobacter sp. DMC5

Annotation: putative GTPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00750: LAO/AO transport system ATPase
  • amino acids 18 to 322 (305 residues), 373 bits, E=4.9e-116
• PF03308: MeaB
  • amino acids 23 to 293 (271 residues), 392.2 bits, E=4.9e-122

Best Hits

Swiss-Prot: 61% identical to Y1496_MYCTU: Probable GTPase Rv1496 (Rv1496) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K07588, LAO/AO transport system kinase [EC: 2.7.-.-] (inferred from 90% identity to xau:Xaut_2490)

MetaCyc: 51% identical to methylmalonyl-CoA mutase-interacting GTPase YgfD (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"putative periplasmic protein kinase ArgK and related GTPases of G3E family"

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Lipopolysaccharide biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.-.-, 3.6.1.15

Use Curated BLAST to search for 2.7.-.- or 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (328 amino acids)

>GFF4245 putative GTPase (Xanthobacter sp. DMC5)
MTAAEHNDEKLAAGVARGERAALARAITLVESRRSDHRRRAQALLQSLLPKTGHAYRIGI
TGVPGVGKSTTIDALGTYLTGLGHRVAVLAVDPSSTRTGGSILGDKTRMARLSVDPAAFI
RPSPSGGTLGGIAAKTRETMLLCEAAGFDVVLVETVGVGQSETAVADLTDTFLVLMLPGA
GDELQGIKKGVLELADIVAVNKADGDNINRARAAAGEYRTALHLLGAREAHWSPPVLTYS
ALTGDGIPGVWEQVVLHRQKCEAAGTFAATRRAQQVRWMWTLLNEHLAEKVAHDPGVKAR
LPALEREVAEGALSPALAAAEIAGLIGL