Protein Info for GFF4244 in Pseudomonas sp. DMC3

Annotation: CRP-like cAMP-activated global transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 225 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00027: cNMP_binding" amino acids 31 to 117 (87 residues), 75.5 bits, see alignment E=2.6e-25 PF13545: HTH_Crp_2" amino acids 150 to 222 (73 residues), 55 bits, see alignment E=6.4e-19

Best Hits

Swiss-Prot: 31% identical to CRPL_MYCTU: CRP-like cAMP-activated global transcriptional regulator (crp) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_0199)

Predicted SEED Role

"transcriptional regulator, Crp/Fnr family" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (225 amino acids)

>GFF4244 CRP-like cAMP-activated global transcriptional regulator (Pseudomonas sp. DMC3)
MQIRARLLSGQWFSHLPASFQDSLLALARERRLTAGQRLFQRGDAPCGLYAVLDGAVRIG
AVSEQGKEALLSLVEAPHWFGEICLFDGQPRTHDAYAVGPCTLLNIPQAALLKLLDEQPQ
YWRHLALLMSHKLRLAFINLEQLSLLPAPARLVHRLLMIAEGYGELDAPRRVLQLPQEQL
AAMLSLSRQTTNQILKDLQGQGIIGLAYGEIEILDAARLRALATI