Protein Info for GFF4241 in Variovorax sp. SCN45

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1008 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 813 to 831 (19 residues), see Phobius details PF12860: PAS_7" amino acids 332 to 442 (111 residues), 58.8 bits, see alignment E=1.5e-19 PF08448: PAS_4" amino acids 457 to 566 (110 residues), 34.6 bits, see alignment E=5.1e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 571 to 739 (169 residues), 145.3 bits, see alignment E=1.4e-46 PF00990: GGDEF" amino acids 575 to 736 (162 residues), 161.4 bits, see alignment E=3.9e-51 PF00563: EAL" amino acids 758 to 992 (235 residues), 223.9 bits, see alignment E=4.9e-70

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1008 amino acids)

>GFF4241 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Variovorax sp. SCN45)
VKPHFPRRATAIVYGVAVLFLATVAAVASLIIWDGRQKAISDHENLAVRFVSGSEAALNR
SLLSVDMLLAGANELLRGATVGSAGVELGRFEDHRLLDNVVRQNLLVRHIAFVDARGVVV
ASSDRRGARLPLALPAGFIDSVLANPVSTLAISAPTISLVTSQKVLFFARAATLADGSRM
VAVAEVQLSLLATIMTQGAGIRGLEVTLERDGGPLLASLPPRDDLDGKRIEPALHEQTSD
GKPSRMAARLSGLPAIVVARPTLHRNLLIVASIPLTAALRDWRRERDLIAWTAVAIGLMI
LAVARFTHIQLRRQWRAQMELLRSKSTLDQALESMIDGFILLDSDNRVLTWNRGFVAMFP
WVAPMIAPQLPFRRIVDETARHVVHEQGPADWTARNLPQMELPYSEQEALLQGGQVIRAA
RSRTPDGGIVCVYQDITEKRRHMAAIIESKAQLQATLDALPDLLLEVGLDGRCHGYHAPR
APVPAIDVENPVGHTLSELLPAEAVAQVMAALRDADASGHSAGRQFERRHAHDKDWFEIS
VSRKSIGAGEDARFIVILRNITESKRAAREIEHLAFYDTLTGLPNRRLLLDRLKRAIGAT
ARGSRRCALLFLDLDNFKTLNDALGHDIGDVMLKQVAQRLQDCLREGDMVARLGGDEFVV
ILEDLSTQTVVATSQTETMGAAILARLNKPYQLGLQQHHSTCSIGAALFDAKNASMEEVL
KQADIAMYYAKTDGGNALRFFEAGMQTTITARATLESELHTAIADRQFVLHFQSQVTHEN
KVTGAEVLVRWRHPVRGLLLPHEFIDVAEDTGLIIPLGLWVLETACAQLMAWRDDPRRRH
LQLSVNVSARQFREQDFVDQVRLVFKRTGADPRKLKLELTESLLHEKVIDAIGKMNALAA
LGIQFSMDDFGTGYSSLSYMTQLPLSQIKIDKFFVHSIGLNPKVELIIQTIIGMARNLEL
QIVAEGVETQAQRDFLERHGCRVCQGYLFGRPQPLQVYEGELDFDTAA