Protein Info for GFF4229 in Xanthobacter sp. DMC5

Annotation: Methanesulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 25 to 370 (346 residues), 616.3 bits, see alignment E=7e-190 PF00296: Bac_luciferase" amino acids 26 to 346 (321 residues), 263.5 bits, see alignment E=1.5e-82

Best Hits

Swiss-Prot: 71% identical to SSUD_RHOPA: Alkanesulfonate monooxygenase (ssuD) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 88% identity to xau:Xaut_1213)

MetaCyc: 67% identical to alkanesulfonate monooxygenase subunit (Pseudomonas putida DS1)
RXN-18086 [EC: 1.14.14.34]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.34 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (407 amino acids)

>GFF4229 Methanesulfonate monooxygenase (Xanthobacter sp. DMC5)
MTSPASFFAASTVADPTLPAATEANVLWFLPTHGDGHYLGTSHKAREVSLGYLRQIAQAA
DQLGYFGVLIPTGRSCEDSWIVASALAGLTERLRFLVAVRPGLLSPAVAARMTATLDRIS
GGRALINVVTGGDPVENKGDGFFADHAERYEITAEFLDIYTRLLKGEDVNFEGKHFKVEG
GKLLYPPVQQPHPPLFFGGSSAPAHDVAARQVQKYLTWGEPPAQVAEKIRDIRARAAALG
REVTFGVRLHVIVRDTAAEAWAAADDLIRYVDDATIAEAQKVFSRMDSEGQRRMSALHGG
RRDKLEVAPNLWAGVGLVRGGAGTALVGDPETVAERLKEYMAVGVDTFVLSGYPHLEEAY
RFGESVLPLLPRAAGTGAPGVVTNHRGPFGEIIGNAIVPEARRVSAS