Protein Info for GFF4222 in Xanthobacter sp. DMC5

Annotation: Monoterpene epsilon-lactone hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 PF10340: Say1_Mug180" amino acids 70 to 197 (128 residues), 47.9 bits, see alignment E=1.4e-16 PF20434: BD-FAE" amino acids 74 to 164 (91 residues), 33.9 bits, see alignment E=3.7e-12 PF07859: Abhydrolase_3" amino acids 78 to 278 (201 residues), 195.2 bits, see alignment E=1.8e-61

Best Hits

KEGG orthology group: None (inferred from 42% identity to bbr:BB4484)

Predicted SEED Role

"putative esterase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (308 amino acids)

>GFF4222 Monoterpene epsilon-lactone hydrolase (Xanthobacter sp. DMC5)
LAEALSSGAPAFERVRARIEAATSAWAAGLTLAEIRDSFEAFLGAGEGAPEVRRTPLTLA
GLPACAFDAPGGDAARVLLYCHGGGFQIGSIRSHAGLMARIALASGMRVIGFDYRLAPEH
RFPAAHDDAFAVYRALVAAGETPLALVGDSAGGALALGTALRARDAGLPLPKALVLLSPW
LDLTMSGESYTALAEKDIFSRPEQLKAMARTYLGRDNRPDDPAASPVLADLSGLPPVLVH
AGGHDITLDDSHLLARRAGEQSGRVEVEVFPGMCHHFQVFEELPEAAQSLARIGTFLRAV
NPGLLPTP