Protein Info for PS417_02150 in Pseudomonas simiae WCS417

Annotation: potassium/proton antiporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 32 to 54 (23 residues), see Phobius details amino acids 60 to 77 (18 residues), see Phobius details amino acids 88 to 113 (26 residues), see Phobius details amino acids 119 to 142 (24 residues), see Phobius details amino acids 163 to 184 (22 residues), see Phobius details amino acids 190 to 209 (20 residues), see Phobius details amino acids 217 to 236 (20 residues), see Phobius details amino acids 242 to 260 (19 residues), see Phobius details amino acids 272 to 290 (19 residues), see Phobius details amino acids 297 to 322 (26 residues), see Phobius details amino acids 334 to 355 (22 residues), see Phobius details amino acids 362 to 384 (23 residues), see Phobius details PF00999: Na_H_Exchanger" amino acids 19 to 387 (369 residues), 197.1 bits, see alignment E=6.6e-62 PF02080: TrkA_C" amino acids 417 to 482 (66 residues), 51.2 bits, see alignment E=1.5e-17 PF03471: CorC_HlyC" amino acids 495 to 568 (74 residues), 41.9 bits, see alignment E=1.2e-14

Best Hits

Swiss-Prot: 100% identical to NHAP2_PSEFS: K(+)/H(+) antiporter NhaP2 (nhaP2) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K11105, cell volume regulation protein A (inferred from 100% identity to pfs:PFLU0446)

Predicted SEED Role

"Na(+)/H(+) antiporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TVL3 at UniProt or InterPro

Protein Sequence (580 amino acids)

>PS417_02150 potassium/proton antiporter (Pseudomonas simiae WCS417)
MNATTINSLFLIGALLVSASILVSSLSSRLGIPILVIILAVGMVAGVDGGGIIFDNYPTA
YLVGNLALAVILLDGGLRTRVASFRVALWPALSLATVGVLITTGLTGMAAAWLFDLNLIQ
GLLIGAIVGSTDAAAVFSLLGGKGLNERVTASLEIESGSNDPMAVFLTVTLIDMLASGQT
GLHWSLLTHLVREFGIGGVVGLGGGWLMLQMVNRINLATGLYPILVIAGGLFVFAVTNAL
HGSGFLAVYLCGLVIGNRPVRSRHGILHMLDGMAWLAQIGMFLVLGLLVTPHDLLPIALP
ALGLALWMILFARPLSVMVGLLPFKAFHGREKAFIAWVGLRGAVPIILAVFPLMAGLPHA
QLYFNLAFFIVLVSLLVQGTSLPWVAKLLKVTVPPDPAPISRAALEVHVTSEWELFVYRL
GAEKWCIGAALRELKMPEGTRIAALFRGQQLLHPSGSTVLEVGDLLCVIGHEHNLPALGK
LFSQAPQRGLDLRFFGDFVLEGDAQLGAVSALYGLKLEGIDPDMPLSSFITQKVGGAPIV
GDQVEWNNTIWTVAVMDGNKIGKVGVRFPEGSRPGPGLFL